FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102830
Sequences flagged as poor quality0
Sequence length197-201
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG1208311.750461927453077No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA1160111.281727122435086No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG1052710.23728483905475No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTGTTATCCCAGGA81067.88291354663036No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA65506.369736458232033No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGGCCAAAA51515.009238549061558No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG46484.520081688223281No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC45144.389769522512886No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGCGTCAGTTGTACCTTAGTA44874.363512593601089No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG36153.5155110376349317No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG27602.6840416220947194No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG25952.5235826120781875No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC16311.5861130020422058No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTCAGCGTCAGTATCTGTCCAGTA9350.9092677234270155No Hit
CCTGTTTGCTACCCACGCTTTCGGGCATGAACGTCAGTGTTATCCCAGGA8720.8480015559661578No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTTTCGTCCAGCA6630.6447534766118838No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG6580.6398910823689585No Hit
CCTGTTTGCTCCCCCGGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG5020.4881843819896917No Hit
CCTGTTTGATCCCCACGCTTTCGAGCCTCAACGTCAGTTATTGTCCAGTA4620.44928522804628995No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACACGCCAGAG4330.4210833414373238No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTACACTCCCGTA4080.39677137022269765No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG4000.3889915394340173No Hit
CCTGTTCGATACCCGCACCTTCGTGCTTAAGCGTCAGTAACGTTACGGTA3930.38218418749392197No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTAACACTCCCGTA3660.3559272585821258No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGTGTCAGTTGCAGTCCAGAA3420.33258776621608477No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGTGTCAGTATCTGTCCAGTA3240.315083146941554No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTGAGTTCCAGCA2900.28201886608966253No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC2630.2557619371778664No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACCTTCCCAAGG2550.24798210638918602No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTATTATCCCAGGG2410.23436740250899543No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCATGAACGTCAGTGTTATCCCAGGA2250.21880774093163474No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG2200.21394534668870954No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTGCAGCGTCAGTTTCTGTCCAGTG2080.202275600505689No Hit
CCTGTTCGCTCCCCACGCTTTCGTCTCTCAGCGTCAATTGTCTGTTAGTC1960.19060585432266847No Hit
CCTGTTCGCTCCCCCGGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG1850.179908586988233No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG1820.17699115044247787No Hit
CCTGTTTGATACCCACGCCTTCGTGCTTCAGTGTCAGTCGCAGTATGGCA1810.17601867159389284No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCATCAGCGTCAATTAATTGTTAGTA1770.17212875619955267No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAAGCCAGAG1700.16532140425945735No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTTTGGTCCAGCA1630.15851405231936205No Hit
CCTGTTTGCTCCCCACGCTTTCGTACCTGAGCGTCAGTAAAAGTCCAGAA1580.15365165807643683No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTGTTATCCCAGGG1570.1526791792278518No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCCTCAGCGTCAGTTGTATCTTAGTG1450.14100943304483127No Hit
CCTGTTCGATACCCACGCTTTCGAGCATCAGCGTCAGTTGCGCTACAGTA1450.14100943304483127No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTACATTCCCAAGG1320.1283672080132257No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATTGGCCAGTA1300.12642225031605564No Hit
CCTGTTCGCTCCCCACGCTTTCGTCTCTCAGCGTCAATCGTCTGTTAGTC1290.12544977146747058No Hit
CCTGTTTGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGTGCTCCCGCA1280.12447729261888553No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTGCAGCCCGGAC1280.12447729261888553No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTAAGTTCCAGCA1270.1235048137703005No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCATCAGCGTCAATAAATTGTTAGTA1260.12253233492171545No Hit
CCTGTTTGCTACCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG1210.11766994067879025No Hit
CCTGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA1170.11378002528445005No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGTA1160.11280754643586502No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA1150.11183506758727997No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTAACGCTCCCGCA1050.10211027910142954No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGCGG151.9878134E-6407.17212195
GTCACCG7700.0407.17212195
CTGGCCG600.0407.17212195
CGAACAA151.9878134E-6407.17212195
CATGCGG151.9878134E-6407.17212195
CCAACCG400.0407.1721195
AGGGCCG103.214415E-4407.1721195
ATAGCCG103.214415E-4407.1721195
GCGCCCG103.214415E-4407.1721195
GTAACCG10200.0407.1721195
GGAACCG2300.0407.1721195
CAAACGG103.214415E-4407.1721195
ATATCCG103.214415E-4407.1721195
TAAACCA6050.0386.98172195
GCAACCG600.0373.2411195
AAGGCCG750.0352.88248195
ATAACCG351.8189894E-12349.00467195
CAGACCG650.0344.5302195
CAGGCCG6150.0337.65494195
TAGCCCG1000.0305.3791195
Produced by FastQC (version 0.11.5)