FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107582
Sequences flagged as poor quality0
Sequence length198-201
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA4225339.2751575542377No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA2101019.529289286311837No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA1843917.13948430034764No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA34823.236600918369244No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA24052.23550408060828No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA20601.9148184640553252No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA17751.6499042590767972No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA16261.5114052536669702No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGGGCGCA14531.3505976836273728No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGAGCGCA11571.0754587198601997No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCTTGTA7680.713874068152665No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA6380.5930360097414066No Hit
TACGTATGTCGCAAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA5730.5326169805357774No Hit
TACGTAGGGCGCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA3790.35228941644513023No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGCGCA3460.3216151400791954No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA3220.29930657544942463No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGAGCTTGTA2680.24911230503244036No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA2640.24539421092747857No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGAGCTTGTA2070.19241136993177296No Hit
TACGTAGGTCCCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA1970.1831161346693685No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA1890.1756799464594449No Hit
TACGGAGGGTGCAAGCGTTACTCGGAATCACTGGGCGTAAAGGACGCGTA1670.155230428882155No Hit
TACGGAGGATGCGAGCGTTATCCGGAATCATTGGGTTTAAAGGGTCCGTA1620.15058281125095277No Hit
TACGTAGGGTGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGGGCTCGTA1310.12176758193749883No Hit
TACGTAGGTCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA1300.12083805841125839No Hit
TACGTAGGGCGCGAGCGTTGTCCGGATTTATTGGGCGTAAAGAGCTTGTA1290.11990853488501793No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATCATTGGGCGTAAAGGGAGCGCA1270.11804948783253703No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA1260.11711996430629659No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGCGCA1240.1152609172538157No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA1160.1078247290438921No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGCCG106.9410703E-4314.98947195
AAGATAG750.0314.98947195
GCAGGCA253.5470293E-10314.98944195
AGGCAGG1550.0314.98944195
AAGGCGG2600.0278.6445195
AAGGCAG11050.0265.10428195
AAGCGGG6450.0241.73608195
AGGCGAG201.749461E-5236.2421195
GAAGGCA41450.0231.77753195
GAGGGCG900.0227.4924195
TCCCGAG42900.0194.616949
GTCACGA153.796775E-5194.616938
GTCACAA153.796775E-5194.616938
TAGTGCG153.796775E-5194.616935
GTCGCAA600.0194.616938
GGTCCCG42850.0194.616937
TATGTCG650.0194.616935
GGTCCAG3000.0194.616938
TACGGAG2700.0194.616931
GATGCGA308.367351E-11194.616938
Produced by FastQC (version 0.11.5)