FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences237517
Sequences flagged as poor quality0
Sequence length194-201
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG5157321.713393146595823No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA4813320.265075762998016No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG3446514.51054029816813No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGTGCTCCCGTA210688.870101929546095No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG123865.214784625942563No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTGATCGTCCAGAA114024.800498490634355No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG42801.8019762795926189No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTGTCGTCCAGTA36461.5350480176155812No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTACACTCCCGTA33751.420950921407731No Hit
CCTGTTTGCTCCCCTCGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG29141.2268595511058156No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTTTCGTCCAGCA25371.0681340704033817No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG20670.8702534976443792No Hit
CCCGTTCGCTCCCCTGGCTTTCGAGCCTCAGCGTCAGTTGCAGTCCAGAA17340.7300530067321497No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC16510.6951081396279003No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG16470.6934240496469727No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTGAGTTCCAGCA15210.6403752152477507No Hit
CCTGTTCGCTCCCCCGGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG14250.5999570557054863No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG13200.5557496937061347No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGTA12660.533014478963611No Hit
CCTGTTTGATCCCCACGCTTTCGAGCCTCAACGTCAGTTATTGTCCAGTA11830.49806961185936166No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTTTGGTCCAGCA10250.43154805761271825No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG8040.3385020861664639No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC7740.32587141130950625No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG5050.21261636009211973No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTCCGTCCAGAA4740.1995646627399302No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTGCAGCCCGGAC4600.19367034780668332No Hit
CCTGTTTGCTCCCCTAGCTTTCGCTCCTCAGCGTCAGTTATGGCCCAGAA4480.18861807786390028No Hit
CCCGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG4340.18272376293065337No Hit
CCCGTTCGCTCCCCTCGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA4290.1806186504544938No Hit
CCTGTTTGATACCCGCACCTTCGCGCTTCAGCGTCAGTTGTGCTCCCGTA4150.1747243355212469No Hit
CCTGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA3890.16377775064521696No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAA3220.1355692434646783No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGTGCTCCCGTA3120.1313590185123591No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG2990.12588572607434415No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG2820.11872834365540151No Hit
CCTATTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCTTTGGCTAGCA2660.1119919837316908No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACCG650.0433.8267195
ATGGCCG151.5481255E-6433.8267195
ATTGCCG900.0433.82666195
TAATCCG1450.0433.82666195
GGAACCG450.0433.82666195
CTGGCCG53600.0432.20792195
TCCACGG23750.0427.43344195
GTCACCG1550.0419.8323195
CCAGCCG700.0402.83905195
GAAGCCG3200.0393.15543195
CACTCCG1300.0383.76978195
TTCGCCG4100.0359.75873195
TCTGCGG252.7372153E-8347.06134195
AAGGCCG1700.0293.471195
CGGGCCG158.970623E-4289.2178195
CCGGCCG158.970623E-4289.2178195
CATTCCG455.456968E-12289.21777195
ATTTCCG302.4633662E-5216.91335195
TATTCCG200.002122704216.91333195
ATCCCCA1700.0194.44579
Produced by FastQC (version 0.11.5)