FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301452

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301452
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97326
Sequences flagged as poor quality0
Sequence length197-201
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA1352413.89556747426176No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC1020510.485379035406778No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG58756.0364137024022355No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG50125.149703059819576No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTGTTATCCCAGGA49255.060312763290385No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG45834.708916425210118No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGCGTCAGTTGTACCTTAGTA34333.5273205515484047No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC28922.971456753591024No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG27852.861516963606847No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG27702.8461048435156076No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG26632.7361650535314306No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA25382.607730719437766No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTACACTCCCGTA24662.533752542999815No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGGCCAAAA21922.2522244826665023No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG21012.158724287446314No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTTTCGTCCAGCA15421.5843659453794463No Hit
CCTGTTCGATACCCGCACCTTCGTGCTTAAGCGTCAGTAACGTTACGGTA13371.3737336374658364No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC12911.326469802519368No Hit
CCTGTTCGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTATCGTCCAGTG12111.2442718286994225No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGTA9931.0202823500400715No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG9550.9812383124755976No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTGATCGTCCAGAA9020.9267821548198838No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA8150.8373918582906932No Hit
CCTGTTCGCTCCCCCGGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG7900.8117049914719602No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTGCAGCCCGGAC7890.8106775167992109No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGTGCTCCCGTA7810.8024577194172164No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTATCGTCCAGTA6160.6329243984135791No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTGTCGTCCAGTA6080.6247046010315845No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAA6010.6175122783223393No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGAA5990.6154573289768407No Hit
CCTGTTTGCTCCCCTAGCTTTCGCTCCTCAGCGTCAGTTATGGCCCAGAA4700.48291309619217887No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTGAGTTCCAGCA4020.4130448184452253No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGTGTCAGTTGCAGTCCAGAA3220.33084684462528No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTAACGCTCCCGCA3190.32776442060703204No Hit
CCTATTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCTTTGGCTAGCA2760.2835830096788114No Hit
CCTGTTCGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCACTCCAGAC2090.2147422066046072No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC2010.20652240922261264No Hit
CCGGTTCGCTCCCCACGCTTTCGTGCCTTAGCGTCAGAAATGGCCCAGTA1950.20035756118611678No Hit
CCTGTTTGATCCCCACGCTTTCGAGCCTCAACGTCAGTTATTGTCCAGTA1920.19727513716786882No Hit
CCCGTTCGCTCCCCTGGCTTTCGAGCCTCAGCGTCAGTTGCAGTCCAGAA1700.17467069436738386No Hit
CCTGTTCGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCACTCCGGAC1650.16953332100363727No Hit
CCTGTTTGCTCCCCTCGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG1580.16234099829439205No Hit
CCTGTTTGCTCCCCACGCTTTCGTACCTGAGCGTCAGTAAAAGTCCAGAA1550.1592585742761441No Hit
CCTGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA1410.14487392885765366No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTCAGCGTCAGTATCTGTCCAGTA1340.13768160614840844No Hit
CCTGTTTGATACCCGCACCTTCGCGCTTAAGCGTCAGTTGCGCTCCCGTC1320.1356266568029098No Hit
CCTGTTTGCTACCCACGCTTTCGGGCATGAACGTCAGTGCTATCCCAGGG1300.13357170745741118No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCATCAGCGTCAATTAATTGTTAGTA1270.13048928343916322No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACCTTCCCAAGG1250.1284343340936646No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTATCGTCCAGTT1160.11918706203892075No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGTGTCAGTTATGCCTTAGTA1110.11404968867517416No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTATCGTCCAGTG1080.1109672646569262No Hit
CCTGTTTGCTCCCCAAACTGTCGTCCCTCATCGTCAAGTATTCTATAGTT1010.103774941947681No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTCCG106.0120976E-4330.42758195
GAAGCCG1100.0330.42758195
TCAACCG106.0120976E-4330.42758195
GACACCG106.0120976E-4330.42758195
CTGGCCG4500.0330.42758195
CCACCCG154.5810975E-6330.42758195
TCTGCGG154.5810975E-6330.42758195
ATTGCCG203.4833647E-8330.42758195
TCGTCCG301.8189894E-12330.42758195
ACAACCG106.0120976E-4330.42758195
CATGCCG106.0120976E-4330.42758195
GCAACCG1450.0330.42755195
TAATCCG500.0330.42755195
GTAACCG5900.0330.42755195
CAAACGG1750.0330.42755195
GTCACCG5050.0323.88446195
CCAACCG2500.0317.21045195
GGAACCG700.0306.82562195
CCAGCCG950.0295.64572195
CAAGCCG1250.0251.12494195
Produced by FastQC (version 0.11.5)