FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132787
Sequences flagged as poor quality0
Sequence length197-201
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA1786513.45387726208138No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC1584911.935656351901919No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG121829.17409083720545No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG78585.917747972316567No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC68045.123995571855679No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG60734.573489874761837No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG57084.298613569099385No Hit
CCCGTTCGCTCCCCTGGCTTTCGAGCCTCAGCGTCAGTTGCAGTCCAGAA45833.4513920790438823No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGTGCTCCCGTA44333.3384292137031486No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTGCTGTCCAGTG41973.160700972233728No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG41483.1237997695557547No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTGTTATCCCAGGA35762.6930347097230904No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTACACTCCCGTA24561.849578648512279No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTATCGTCCAGTA23251.750924412781372No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA18461.3901963294599622No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGTA16501.2425915187480703No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG14641.1025175657255604No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTGAGTTCCAGCA14361.0814311641952903No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTTTCGTCCAGCA10910.8216165739116028No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTAACGCTCCCGCA10710.8065548585328384No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG10580.7967647435366414No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTGTCGTCCAGTA9620.7244685097185719No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG7170.5399624963287069No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGTGTCAGTTGCAGTCCAGAA7090.5339378101772011No Hit
CCTGTTTGCTCCCCCGGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG6730.506826722495425No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGGCCAAAA6530.49176500711666055No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC5930.446579860980367No Hit
CCTGTTCGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCACTCCGGAC5280.39762928599938246No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC5160.3885922567721238No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGAA4770.359221911783533No Hit
CCTGTTTGATCCCCACGCTTTCGAGCCTCAACGTCAGTTATTGTCCAGTA4320.3253330521813129No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTGCAGCCCGGAC3990.3004812218063515No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTATCGTCCAGTT3660.2756293914313901No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGTGCTCCCGTA3220.24249361759810825No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG3160.23797510298447894No Hit
CCTGTTCGATACCCGCACCTTCGCGCTTCAGCGTCAGTTGCGCTCCCGTC3050.22969115952615843No Hit
CCTGTTTGCTCCCCTAGCTTTCGCTCCTCAGCGTCAGTTATGGCCCAGAA2860.21538252991633217No Hit
CCTGTTCGATACCCGCACCTTCGAGCCTCAGCGTCAGTTACGCTCCGGAT2670.2010739003065059No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAA2610.19655538569287653No Hit
CCTATTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCTTTGGCTAGCA2600.19580229992393833No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTTTGGTCCAGCA2460.1852590991588032No Hit
CCTGTTTGCTCCCCTCGCTTTCGCACTTCAGCGTCAGTTGCTGTCCAGTG2340.17622206993154452No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGTGTCAGTTATGCCTTAGTA2310.17396281262472982No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG2180.1641726976285329No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAAGCCAGAG1940.14609863917401553No Hit
CCGGTTCGCTCCCCACGCTTTCGTGCCTTAGCGTCAGAAATGGCCCAGTA1890.1423332103293244No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGCGTCAGTTGTACCTTAGTA1850.1393208672535715No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG1730.13028383802631283No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAGCGTCAGTTGTTGCCCAGAA1670.12576532341268348No Hit
CCCGTTCGCTCCCCTGGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAA1660.12501223764374525No Hit
CCTGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA1600.12049372303011589No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTGCAGTCCAGAA1540.11597520841648656No Hit
CCTGTTTGATACCCGCACCTTCGCGCTTAAGCGTCAGTTGCGCTCCCGTC1380.10392583611347496No Hit
CCCGTTCGCTCCCCTCGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA1380.10392583611347496No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCCG4300.0437.1262195
CTGGCCG1250.0437.12616195
TCTGCGG151.4985872E-6437.12616195
TAATCCG1400.0437.12616195
TCGTCCG500.0437.12616195
GGAACCG450.0437.12616195
ATTTCCG300.0437.12616195
GTAACCG4550.0432.32257195
CCAGCCG1050.0416.31064195
GTCACCG800.0409.80582195
GAAGCCG650.0403.5011195
CCAACCG1650.0397.38745195
TCCACGG5750.0395.3141195
ATTGCCG400.0382.4854195
TCTCCGG6600.0377.51807195
CAAGCCG3900.0375.48016195
GCAACCG750.0349.70093195
AAGGCCG700.0343.45627195
TCTCCAG26300.0300.83588195
TCTCCCG158.759488E-4291.41745195
Produced by FastQC (version 0.11.5)