FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301460

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301460
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93128
Sequences flagged as poor quality0
Sequence length198-201
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1945720.892749763766No Hit
CCTGTTTGCTCCCCATGCTTTCGTACCTCAGCGTCAGTATTAGGCCAGAT1210512.99823898290525No Hit
CCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAA1189512.772742891504166No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGTGTCAGTATGATGCCAGGG84749.09930418348939No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG58216.250536895455718No Hit
CCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAC54525.854308049136672No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG43934.717163473928357No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCTTGGCA40434.341336654926552No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG33923.6422987715831976No Hit
CCTGTTTGCTACCCATGCTTTCGCTCCTCAGCGTCAGTAACTGCCCAGTA28023.008762133837299No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA22862.4546860235374965No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG16251.7449102310798041No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT12081.2971394210119405No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCTTGGGA4070.4370329009535263No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCTTTGCA3200.34361309165879217No Hit
CCTGTTTGATCCCCACGCTTTCGCACCTCAGCGTCAGTTACAGACCAGAA2240.24052916416115455No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG2170.23301262778111848No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCTTGGAA1590.17073275491796236No Hit
CCTGTTTGATCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1300.13959281848638433No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAA1300.13959281848638433No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1150.12348595481487844No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCCG100.0013537525252.02571195
CTGGCCG100.0013537525252.02571195
CGGGCCG1350.0252.02571195
CGAACCG2300.0252.02571195
AAGGCCG850.0252.02571195
CAAACCA2050.0252.02571195
ACAACCG100.0013537525252.02571195
AAAACCG17400.0251.30151195
GAAACCG15000.0251.18564195
ATAACCA9850.0250.74637195
AAAGCCG13950.0247.50912195
TGAACCA1250.0241.94469195
TTTGCTC40150.0194.771775
TTGCTCC40150.0194.771776
ATCCCCA19400.0194.771769
GTTTGAT21800.0194.771764
CCTGTTC17550.0194.771761
TGATCCC19350.0194.771767
TGTTCGC17450.0194.771763
TGTTCGA100.0029291548194.771763
Produced by FastQC (version 0.11.5)