FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301466

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301466
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7703
Sequences flagged as poor quality0
Sequence length198-201
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACAGGCCCAGGG99812.955991172270545No Hit
CCGGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGAAGTGGACCAGGT98712.813189666363755No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGCAGGCCCAGGG7439.645592626249513No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTGTTATCCCAGGA5597.256912891081396No Hit
CCTGTTTGCTCCCCATGCTTTCGCACCTCAGTGTCAGTATTAGGCCAGAT1942.5184992859924704No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1521.973257172530183No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG1371.7785278462936518No Hit
CCTGTTTGCTCCCCACGCTTTCGCATCTGAGTGTCAGTATCTGTCCAGGG1141.4799428793976372No Hit
CCTGTTTGATCCCCGCACTTTCGAGCCTCAGTGTCAGTTACAGTTTTGTG981.2722315980786707No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG971.2592496429962352No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTGCTGGTCCAGGT901.1683759574191872No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG871.1294300921718812No Hit
CCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAA861.1164481370894457No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG851.1034661820070104No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACTTGTCCAGCG841.090484226924575No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAACGGACCAGTA791.0255744515123977No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGTGTCAGTATGATGCCAGGG720.9347007659353498No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA710.9217188108529145No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT660.8568090354407374No Hit
CCCATTTGCTCCCCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCA630.8178631701934311No Hit
CCTGTTTGCTCCCCATGCTTTCGTACCTCAGCGTCAGTATTAGGCCAGAT610.7918992600285603No Hit
CCTGTTCGCTACCCATGCTTTCGCTCCTCAGCGTCAGTAACTGCCCAGTA550.7140075295339477No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCTCGTCCCAGGG540.7010255744515124No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCGCATCCCAGGG540.7010255744515124No Hit
CCGGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCACAAATCGTCCAGGT520.6750616642866416No Hit
CCTGTTTGCTACCCACGCTTTCGAATCTCAGTGTCAATATTATGCCAGGA510.6620797092042062No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACCAGTCCAGTG500.6490977541217707No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA490.6361157990393354No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTAATGAGCCAGTA480.6231338439568999No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG470.6101518888744645No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCGAGCCAGTG440.5712060236271582No Hit
CCCATTCGCTCCCCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCA440.5712060236271582No Hit
CCTGTTTGCTACCCACGCTTTCGGGCATGAACGTCAGTGTTATCCCAGGA400.5192782032974166No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTATCGACCCAGAA400.5192782032974166No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAATTACAGACCAGAA380.49331429313254577No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTAACAGCCCAGAG380.49331429313254577No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGAAGTGGACCAGGT360.4673503829676749No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG350.4543684278852395No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTTACAGCCCAGAG350.4543684278852395No Hit
CCTGTTTGCTACCCATGCTTTCGCTCCTCAGCGTCAGTAACTGCCCAGTA320.4154225626379333No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATCGGACCAGTT290.37647669739062706No Hit
CCTGTTCGCTCCCCACGCTTTCGTCTCTCAGCGTCAATTGTCTGTTAGTC280.3634947423081916No Hit
CCCGTTTGCTCCCCTGGCTTTCGTGCCTCAGCGTCAGGAAATGTCCAGAG280.3634947423081916No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTCACCGTCCAGAA280.3634947423081916No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTATTGGCCCAGGG270.3505127872257562No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG270.3505127872257562No Hit
CCCGTTCGCTCCCCACGCTTTCGCACATGAGCGTCAGAAAAATGCTAGGG230.29858496689601455No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACAGGTCCAGGG220.2856030118135791No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGACCAGTT210.27262105673114373No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG200.2596391016487083No Hit
CCTGTTCGCTACCCATGCTTTCGCTCCTCAGCGTCAGTATCGGCCCAGAG200.2596391016487083No Hit
CCTGTTTGATCCCCACGCTTTCGAGCCTCAACGTCAGTTATTGTCCAGTA190.24665714656627288No Hit
CCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGAG190.24665714656627288No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC180.23367519148383745No Hit
CCCGTTTGCTCCCCTGGCTTTCGCTCCTCAGTGTCAATGCCGGTCCAGGA180.23367519148383745No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTTTTGCCCCAGGG170.22069323640140204No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGTA170.22069323640140204No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTTACTGCCCAGAG160.20771128131896666No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTCAGCGTCAGTATCTGTCCAGTA160.20771128131896666No Hit
CCGGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCACCAAATGTCCAGTC160.20771128131896666No Hit
CCTGTTTGCTACCCACGCTTTCGAACCTCAGTGTCAGTATGATGCCAGGA160.20771128131896666No Hit
CCTGTTCGCTACCCATGCTTTCGCTCCTCAGCGTCAGTTACTGCCCAGAG150.19472932623653122No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAATCTTGACCCAGGG150.19472932623653122No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTATCGGGCCAGTG150.19472932623653122No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAATGGCCCAGAG140.1817473711540958No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGGCCAGTA140.1817473711540958No Hit
CCGGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGAAGCGGACCAGGT140.1817473711540958No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTGCAGGCCCAGGG130.1687654160716604No Hit
CCCGTTCGCTACCCATGCTTTCGCGTATCAGTGTCAATCTGAGGCCAGTT130.1687654160716604No Hit
CCTGTTTGCTCCCCACGCTGTCGCGCCTCAGCGTCAGTAATGGACCAGCT130.1687654160716604No Hit
CCGGTTCGCTCCCCACGCTTTCGCGTCTCAGCGTCAGGTAAGGCCCAGAA130.1687654160716604No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTACAGGCCCAGGG130.1687654160716604No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTTCAGGCCCAGGG120.15578346098922496No Hit
CCTGTTCGCTCCCCCAGCTTTCGCACTTCAGCGTCAGTTGCCGTCCAGTG110.14280150590678956No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGACCAGTG110.14280150590678956No Hit
CCTGTTTGCTACCCACGCTTTCGTACCTCAGCGTCAGTTTTAGTCCAGGG110.14280150590678956No Hit
CCTGTTTGCTACCTTGGCTTTCGAACATGAGCGTCAGTATCCGTCCAGAA110.14280150590678956No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCATGAGCGTCAGTACAGGCCCAGGG110.14280150590678956No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAAAG110.14280150590678956No Hit
CCTGTTTGCTCCCCTAGCTTTCGCTCCTCAGCGTCAGTTATGGCCCAGAA100.12981955082435415No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGGCCAAAA100.12981955082435415No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTATCCCAGGA100.12981955082435415No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACAGGCCCAGTG100.12981955082435415No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGCCGGCCCAGGG100.12981955082435415No Hit
CCCGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTATCCGTCCAGAA90.11683759574191872No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGCCCAGAA90.11683759574191872No Hit
CCGTTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGAAGTGGACCAGGT90.11683759574191872No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACCGGCCCAGGG90.11683759574191872No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAATCATACCATAGTA90.11683759574191872No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTTACTGCCCAGAG90.11683759574191872No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTGTTATCCCAGTA80.10385564065948333No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACCG151.5223415E-5241.9355195
TTGGCCA151.5223415E-5241.9355195
CAGGCCG850.0241.93549195
GTAACCG1000.0241.93549195
CAAACCA350.0241.93549195
ACAACCG450.0241.93549195
CAGTCCG100.0014964235241.93547195
GAAACCG201.5340629E-7241.93547195
GCAACCG100.0014964235241.93547195
GAGACCG100.0014964235241.93547195
AAAACCG1350.0232.97491195
TGCTCCC5300.0194.80527
TTTGCTC5300.0194.80525
TTGCTCC5300.0194.80526
CGTTCGC307.2759576E-11194.805193
ATCCCCA100.0028758913194.805199
GTTTGCT6600.0194.805194
GTTTGAT204.5905836E-7194.805194
CCTGTTT5800.0194.805191
CCTGTTC351.8189894E-12194.805191
Produced by FastQC (version 0.11.5)