FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301467

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301467
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92932
Sequences flagged as poor quality0
Sequence length199-201
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA4872452.42973356863083No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA1714718.45112555416864No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA1577516.974777256488615No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA29553.1797443291869323No Hit
TACGCAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA16501.775491757414023No Hit
TACGCAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA5570.5993629750785521No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA4500.48422502474927903No Hit
TACGCAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA4460.47992080230706324No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCTTGTA3150.3389575173244953No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA2690.2894589592390135No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGGGAGCGCA1490.16033228597253907No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA1300.13988722937201395No Hit
TACGGAGGGCGCGAGCGTTACCCGGATTTACTGGGCGTAAAGGGCGTGTA1080.11621400593982696No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGAGAGTGCA1010.1086816166659493No Hit
TACGGAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA990.10652950544484138No Hit
TACGTAGGGCGCGAGCGTTGTCCGGATTTATTGGGCGTAAAGAGCTTGTA970.10437739422373349No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCGGG2000.0794.9427195
GAAGGCA18750.0758.9053195
AAGGCAG850.0748.18146195
AAGGCGG750.0688.9503195
GAGGTCC153.8243117E-5194.243295
AGGTGGC4100.0194.243296
TACGCAG2950.0194.243291
AGGTCGC308.367351E-11194.243296
AGGTCCG153.8243117E-5194.243297
GGAGGTC153.8243117E-5194.243294
GGTGCAA153.8243117E-5194.243298
TAGGTCG308.367351E-11194.243295
TAGGTCC66200.0194.243295
TGGCAAG4050.0194.243279
GTCGCGA351.8189894E-12194.243278
GGCGCGA18700.0194.243278
GGTCCCG68650.0194.243277
GCAGGGC500.0194.243274
GGTCCAG100.0029530562194.243278
TACGGAG351.8189894E-12194.243271
Produced by FastQC (version 0.11.5)