FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92932
Sequences flagged as poor quality0
Sequence length197-201
%GC52

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG4577649.25752162871777No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG1725718.569491671329573No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1372814.77209142168467No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA27502.959152929023372No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG19982.1499591098867987No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG14321.5409116343132614No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG4770.5132785262342358No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG3720.40029268712607063No Hit
CCTGTTCGCTCCCCACGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG2830.30452373778676883No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGGCCAAAA2100.2259716782163302No Hit
CCTGTTCGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1790.19261395428915767No Hit
CCTGTTCGCTCCCCACGCTTTCGATCCTCAGCGTCAGTTACAGACCAGAG1520.16356045280420092No Hit
CCTGTTCGATCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1300.13988722937201395No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACTAGAG1260.13558300692979813No Hit
CCTGTTCGCTACCCACGCTTTCGAGCCTCAGCGTCAGTGACAGACCAGAG1190.12805061765592046No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG1080.11621400593982696No Hit
CCTGTTCGCTCCCCACGCTCTCGAGCCTCAGCGTCAGTTACAGACCAGAG990.10652950544484138No Hit
CCGGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGAAGTGGACCAGGT960.10330133861317953No Hit
CCTGTTCGCTACCCATGCTTTCGAGTCTCAGCGTCAGTTGCAGACCAGGT930.10007317178151766No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACCG151.8189894E-1211628.484195
TAAACCA151.8189894E-1211628.484195
AAAACCG300.011628.484195
ACAGCGG55.8624617E-511628.483195
AAACCGG55.8624617E-511628.483195
CCTGCGG55.8624617E-511628.483195
AACCCGG55.8624617E-511628.483195
TCAACCA55.8624617E-511628.483195
TCTCCAG101.2576493E-811628.483195
GGAACCG350.011628.483195
CATTCCG102.344859E-45814.2417195
CGAACCG102.344859E-45814.2417195
CCAGCCG251.9646177E-74651.3936195
CTCACCG209.37843E-42907.1208195
ACTGTTC204.9723894E-7194.01671
CTTTTCG100.002963388194.01672
TGTTGGC153.842153E-5194.01673
TGGTCGC204.9723894E-7194.01673
GTTTGCT17850.0194.01674
GTTTGAT100.002963388194.01674
Produced by FastQC (version 0.11.5)