FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences194192
Sequences flagged as poor quality0
Sequence length197-201
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG3039415.651520145011123No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG2824414.544368460080745No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG2102810.828458432891159No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG1976810.17961605009475No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA140727.246436516437341No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGGCCAAAA109675.647503501689051No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG103665.338015984180604No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG43812.256014665897668No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTCAGCGTCAGTATCTGTCCAGTA38111.9624907308231028No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG35971.852290516602126No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC32991.6988341435280547No Hit
CCTGTTTGATACCCACGCCTTCGTGCTTCAGTGTCAGTCGCAGTATGGCA28531.4691645381890088No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTAACACTCCCGTA27301.40582516272555No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG11610.5978619098624042No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC9820.5056850951635495No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG8000.4119634176485128No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTTACTGCCCAGAG7840.4037241492955425No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGCC7020.36149789898657No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTAACACTCCCGCA6970.35892312762626677No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG6900.3553184477218423No Hit
CCTGTTTGCTACCCACGCTTTCGGGCATGAACGTCAGTGTTATCCCAGGA6800.3501689050012359No Hit
CCTGTTCGATACCCGCACCTTCGAGCCTCAGCGTCAGTTACGCTCCGGAT5990.308457608964324No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTTTCGTCCAGCA5760.29661366070692924No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAAGCCAGAG5710.29403888934662603No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGTGTCAGTTGCAGTCCAGAA5530.2847697124495345No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA5210.26829117574359396No Hit
CCTGTTTGCTCCCCGCACCTTCGTATATCAGCGTCAATCATCGGCCAGAA5200.26777622147153335No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGTGCTCCCGCA5070.261081815934745No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTATGCTCCCGTC5060.26056686166268433No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTGCAGTCCAGAA4960.2554173189420779No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGTGCTCCCGTA3620.18641344648595204No Hit
CCTGTTCGATACCCGCACCTTCGAGCCTAAACGTCAGTTGGGATATGGTA3530.18177885803740626No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTGCTGTCCAGTG3290.16941995550795091No Hit
CCTGTTCGCTACCCACGCTTTCGAGCCTCAGCGTCAGTGACAGACCAGAG3230.16633022987558704No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTACATTCCCAAGG3170.1632405042432232No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGTGTCAGTTATACCTTAGTA3150.16221059569910193No Hit
CCCGTTCGCTCCCCTGGCTTTCGAGCCTCAGCGTCAGTTGCAGTCCAGAA3120.16066573288292No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCCGCA3090.15912087006673806No Hit
CCTGTTTGCTCCCCACGCTTTCGTACCTGAGCGTCAGTAAAAGTCCAGAA3080.15860591579467742No Hit
CCTGTTCGCTCCCCACGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG3050.1570610529784955No Hit
CCTGTTTGCTCCCCGCACCTTCGCACCTCAGCGTCAGTCATCTGCTGGTA2810.1447021504490401No Hit
CCCGTTCGCTACCCTGGCTTTCGCACCTCAGCGTCAGTTACCGTCCAGCA2650.13646288209606985No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG2550.13131333937546347No Hit
CCTGTTTGATCCCCACGCTTTCGAGCCTCAACGTCAGTTATTGTCCAGTA2550.13131333937546347No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGTA2510.1292535222872209No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTAAGTTCCAGCA2500.12873856801516026No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTGCCGTCCAGTG2470.12719370519897832No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACACGCCAGAG2410.1241039795666145No Hit
CCTGTTCGATACCCGCACCTTCGAGCCTCAGCGTCAGTGGTGCTCCCGCA2380.12255911675043256No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTGTCGTCCAGTA2230.11483480266952295No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGACGGAGCCTGGTA2120.10917030567685589No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTACACGGCCCAGA2100.10814039713273461No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTGAGTTCCAGCA1950.10041608305182499No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGCCG350.0803.22687195
AAAGCCA151.3160388E-7803.22687195
GGAACCG3100.0803.22687195
CGACCCG204.129106E-10803.22687195
CCGGCCG700.0803.22687195
GTAACCG900.0803.2268195
ACAACCG251.8189894E-12803.2268195
CATGCGG450.0803.2268195
GTCACCG8150.0793.3713195
CAGACCG3750.0771.0977195
CGAACAA2700.0758.6032195
AGGGCCG600.0736.2913195
ATATCCG500.0722.9042195
TAAACCA11100.0716.3915195
CCAACCG1100.0693.6959195
GAAGCCG251.2569217E-9642.5815195
CGAACCG204.1552266E-7602.42017195
TAATCCG400.0602.42017195
TACGCCA600.0535.48456195
CCAGCCG402.1827873E-11502.01678195
Produced by FastQC (version 0.11.5)