FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences292
Sequences flagged as poor quality0
Sequence length200-201
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGTGTA6020.54794520547945No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA5117.465753424657535No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA3110.616438356164384No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA165.47945205479452No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA103.4246575342465753No Hit
TACGGAGGGAGCTAGCGTTATTCGGAATTACTGGGCGTAAAGCGCACGTA82.73972602739726No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA82.73972602739726No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA72.3972602739726026No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA72.3972602739726026No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA51.7123287671232876No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA51.7123287671232876No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA41.36986301369863No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGCGCA41.36986301369863No Hit
TACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA41.36986301369863No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGTA41.36986301369863No Hit
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGTA31.0273972602739725No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA31.0273972602739725No Hit
TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA31.0273972602739725No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA31.0273972602739725No Hit
TACGGAAGGTTCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA20.684931506849315No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA20.684931506849315No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA20.684931506849315No Hit
TACGTATGTCGCGAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA20.684931506849315No Hit
TACGTATGTCACAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGTCTA20.684931506849315No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGTGTA10.3424657534246575No Hit
TACGTAGGGAGCGAGCGTTATCCGGATTCATTGGGCGTAAAGCGCGCGTA10.3424657534246575No Hit
TACGTAGGTTGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGTA10.3424657534246575No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGAGAGTGCA10.3424657534246575No Hit
TACGTATGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA10.3424657534246575No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA10.3424657534246575No Hit
TACGTAGGGTGCAAGCGTTGTCCGGATTTACTGGGCGTAAAGAGCTCGTA10.3424657534246575No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGTGTA10.3424657534246575No Hit
TACGTATGTCGCAAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA10.3424657534246575No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGGGTGCGCA10.3424657534246575No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA10.3424657534246575No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGTA10.3424657534246575No Hit
TACGTAGGTTCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA10.3424657534246575No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA10.3424657534246575No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA10.3424657534246575No Hit
TACGTAGGGAGCGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTA10.3424657534246575No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGCGTA10.3424657534246575No Hit
TACGGAGGGTGCAAGCGTTACTCGGAATCACTGGGCGTAAAGGACGCGTA10.3424657534246575No Hit
TACAGAGGGTGCGAGCGTTAATCGGAAGTACTGGGCGTAAAGCGAGTGTA10.3424657534246575No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA10.3424657534246575No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGCGTAAAGGGAGCGCA10.3424657534246575No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA10.3424657534246575No Hit
TACGTAGGTCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCTCGTA10.3424657534246575No Hit
TACGTAGGGCGCGAGCGTTATCCGGATTTATTGGGCGTAAAGGGCTTGTA10.3424657534246575No Hit
TACGTAGGGGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA10.3424657534246575No Hit
ATGCAAAATGAGACTCAAAAAGAGGTTGCTGGCATTCAGTCGGCGACTTC10.3424657534246575No Hit
TACGTATGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGTGTA10.3424657534246575No Hit
TACGGAAGGTTCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA10.3424657534246575No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGGGAGCGCA10.3424657534246575No Hit
TACGTAGGGGGCGAGCGTTATCCGGATTCATTGGGCGTAAAGCGCGCGTA10.3424657534246575No Hit
AACGTAGGGTGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA10.3424657534246575No Hit
TACGTATGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA10.3424657534246575No Hit
TCTCGACGCCATTAATAATGTTTTCCGTAAATTCCGCGCCTTCCATGATG10.3424657534246575No Hit
TACAGAGGGTGTGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGTGTA10.3424657534246575No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATGATTGGGCGTAAAGGGTGCGTA10.3424657534246575No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGAGTGCGTA10.3424657534246575No Hit
TACGTAGGGGGCAAGCGTTGTCCGGAATAATTGGGCGTAAAGGGCGCGTA10.3424657534246575No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGCA10.3424657534246575No Hit
TACGTAGGGCGCAAGCTTTGTCCGGAATTATTGGGCGTCAAGAGCGCGTA10.3424657534246575No Hit
TACGGAGGGTCCGAGCGTTGTCCGGATTTATTGGGCTTAAAGCGAGCGTA10.3424657534246575No Hit
TACGTAGGGGGCAAGCGTTATCCGGCTTTACTGGGTGTCAAGGGCTCGCA10.3424657534246575No Hit
TACGTAGGGTGCGAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA10.3424657534246575No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGTGTGCGCA10.3424657534246575No Hit
TACGAAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA10.3424657534246575No Hit
TACGTAGGGGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCGCGTA10.3424657534246575No Hit
TACGTCGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA10.3424657534246575No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGCAG151.474582E-6243.5195
TACGTAG157.3547453E-6194.81
GTGCAAG100.0014141467194.799999
AGGGTGC100.0014141467194.799996
CGTAGGG100.0014141467194.799993
GTAGGGT100.0014141467194.799994
TAGGGTG100.0014141467194.799995
GGGTGCA100.0014141467194.799997
GGTGCAA100.0014141467194.799998
ACGTAGG202.3225493E-5146.099992
Produced by FastQC (version 0.11.5)