FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005301489

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301489
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382
Sequences flagged as poor quality0
Sequence length198-201
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGAAGGATGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGAGCGTA10427.225130890052355No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA359.162303664921465No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA266.806282722513089No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA236.020942408376963No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG143.664921465968586No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA133.4031413612565444No Hit
TACAGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGTGTA112.8795811518324608No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA71.832460732984293No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA61.5706806282722512No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA61.5706806282722512No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA61.5706806282722512No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA61.5706806282722512No Hit
TACGAAGGATGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGAGCGTA61.5706806282722512No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA61.5706806282722512No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGTA51.3089005235602094No Hit
TACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA51.3089005235602094No Hit
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGTA41.0471204188481675No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA30.7853403141361256No Hit
TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA30.7853403141361256No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA30.7853403141361256No Hit
TACGGAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA20.5235602094240838No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA20.5235602094240838No Hit
TACGTAGGGCGCGAGCGTTGTCCGGATTTATTGGGCGTAAAGAGCTTGTA20.5235602094240838No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA20.5235602094240838No Hit
TACGTATGTCGCGAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA20.5235602094240838No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCGCGCA20.5235602094240838No Hit
TACGTAGGGTGCAAGCGTTGTCCGGAATTACTGGGCGTAAAGAGCTCGTA20.5235602094240838No Hit
TACGGAGGGAGCTAGCGTTATTCGGAATTACTGGGCGTAAAGCGCACGTA20.5235602094240838No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGCGCA20.5235602094240838No Hit
ATATCACGAAAATAGTCACGCAAAGCATTGGTATTATCATAAAACGCCTC10.2617801047120419No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA10.2617801047120419No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGTGGAGGGGGGGTG10.2617801047120419No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTATTGGGCGTAAAGCGTGCGTA10.2617801047120419No Hit
TCCATGATGAGACAGGCCGTTTGAATGTTGACGGGATGAACATAATAAGC10.2617801047120419No Hit
CAAGCCTCAATAGCAGGTTTAAGAGCCTCGATACGCTCAAAGTCAAAATA10.2617801047120419No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGTGTA10.2617801047120419No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGA10.2617801047120419No Hit
TACGGAGGATCCGAGCGTTATCCGGCTTTATTGGGTTTAAAGGTAGCGTA10.2617801047120419No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGTGCGGGGGGGGGGGGGG10.2617801047120419No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTCAAGGGAGCGTA10.2617801047120419No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGCGTA10.2617801047120419No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA10.2617801047120419No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA10.2617801047120419No Hit
GCTTTAACCGGACGCTCGACGCCATTAATAATGTTTTCCGTAAATTCAGC10.2617801047120419No Hit
TACGGAGGGCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA10.2617801047120419No Hit
GTACCATACTCAGGCACACAAAAATACTGATAGCAGTCGGCGTGTGAATC10.2617801047120419No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGCGGG10.2617801047120419No Hit
TCTCTTTAATAACCTGATTCAGCGAAACCAATCCGCGGCATTTAGTAGCG10.2617801047120419No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA10.2617801047120419No Hit
TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA10.2617801047120419No Hit
GCTCGCCCTGGTCGTCCGCAGCCGTTGCGAGGTACTAAAGGCAAGCGTAA10.2617801047120419No Hit
GACGAAGGATGCAAGCGTTATCCAGATTCATTGGGTTTAAAGGGAGCGTA10.2617801047120419No Hit
TTTCTGGTCGTGTTCAACAGACCTATAAACATTCTGTGCCGCGTTTCTTT10.2617801047120419No Hit
ATTCTCAAATCCGGCGTCAACCATACCAGCAGAGGAAGCATCAGCACCAG10.2617801047120419No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA10.2617801047120419No Hit
GGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGG10.2617801047120419No Hit
TACGGAGGGAGCTAGCGTTATCCGGAATTACTGGGCGTAAAGCGCACGTA10.2617801047120419No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGG10.2617801047120419No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCTTAAAGCGCGCGCA10.2617801047120419No Hit
TACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCTCGTA10.2617801047120419No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA10.2617801047120419No Hit
ATAGCTTGATGCGGTTATCCATCTGCTTATGGAAGCCAAGCATTGGGGAT10.2617801047120419No Hit
TACGAAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA10.2617801047120419No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGAGCGCA10.2617801047120419No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA10.2617801047120419No Hit
TACGTATGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA10.2617801047120419No Hit
TACGTAGGGTGCGAGCGTTACTCGGAATTACTGGGCGTAAAGCGCGCGCA10.2617801047120419No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTTTAAAGGGAGCGCA10.2617801047120419No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGCGCGTA10.2617801047120419No Hit
TTTGTAGGATACCCTCGCTTTCCTGCTCCTGTTGAGTTTATTGCTGCCGT10.2617801047120419No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTA10.2617801047120419No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGG10.2617801047120419No Hit
TACGTAGGTGGCAAGCGTTGTCCTGATTTTTTGGGCGTTAATCGCGCGCA10.2617801047120419No Hit
CCACCAAGTCCAACCAAATCAAGCAACTTATCAGAAACGGCAGAAGTGCC10.2617801047120419No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA10.2617801047120419No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGTA10.2617801047120419No Hit
CTAGGGACGGCCTCATCAGGGTTAGGAACATTAGAGCCTTGAATGGCAGA10.2617801047120419No Hit
AATAGCAAGGCCACGACGCAATGGAGAAAGACGGAGAGCGCCAACGGCGT10.2617801047120419No Hit
TACGGAAGGTTCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA10.2617801047120419No Hit
CTTGATTTGGTTGGACTTGGTGGCAAGTCTGCCGCTGATAAAGGAAAGGA10.2617801047120419No Hit
CAGGTATATGCACAAAATGAGATGCTTGCTTATCAACAGCAGGAGTCTAC10.2617801047120419No Hit
ATCACCTTGAATGCCACCGGAGGCGGCTTTTTGACCGCCTCCAAACAATT10.2617801047120419No Hit
AAACTTCTTAGACGAATCACCAGAACGGAAAACATCCTTCATAGAAATTT10.2617801047120419No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGCTTAAAGGGAGCGTA10.2617801047120419No Hit
TACGGAAGGGCCAGGCGTTATCCGGATTTACTGGGTTTAAAGGGAGCGTA10.2617801047120419No Hit
TACGGAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA10.2617801047120419No Hit
TACGGGGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGCGCGTA10.2617801047120419No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGCGTA10.2617801047120419No Hit
GTCGAAGGATGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGAGCGTA10.2617801047120419No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGGGCGCA10.2617801047120419No Hit
GACGAAGGCTGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGAGCGTA10.2617801047120419No Hit
TAAAGCTACCAGTTATATGGCTGTTGGTTTCTATGTGGCTAAATACGTTA10.2617801047120419No Hit
TACGGAAGGTCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA10.2617801047120419No Hit
GACGAAGGATGCAAGCGTTATCCGGATTCATTGGGCTTAAAGGGAGCGTA10.2617801047120419No Hit
AATGGCGTCGAGCGTCCGGTTAAAGCCGCTGAATTGTTCGCGTTTCCCTT10.2617801047120419No Hit
GACTATTGACGTCCTTCCCCGTACGCCGGGCAATAATGTTTATGTTGGTT10.2617801047120419No Hit
GCGTCCGGTTAAAGCCGCTGAATTGTTCGCGTTTACCTTGCGTGTACGCG10.2617801047120419No Hit
AATTCCTGCTTTATCAAGATAATTTTTCGACTCATCAGAAATATCCGAAA10.2617801047120419No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGTGG10.2617801047120419No Hit
TTTTAGTAAGCTCTTTTTGATTCTCAAATCCGGCGTCAACCATACCAGCA10.2617801047120419No Hit
TACGTAGGGCGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA10.2617801047120419No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)