FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301494

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301494
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101462
Sequences flagged as poor quality0
Sequence length197-201
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG6610465.15148528513139No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA82898.169561017918038No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG79597.844316098637914No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG43434.280420255859337No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC13611.3413888943643926No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC13591.3394177130354221No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG13491.3295618063905699No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG7710.7598904023181092No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG6720.6623169265340719No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTGATCGTCCAGAA3950.38930831247166425No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTGAGTTCCAGCA3580.3528414578857109No Hit
CCTGTTCGCTACCCACGCTTTCGAGCCTCAGCGTCAGTGACAGACCAGAG3410.3360864165894621No Hit
CCTGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA2830.27892215804931897No Hit
CCTGTTCGCTCCCCACGCTTTCGATCCTCAGCGTCAGTTACAGACCAGAG2660.2621671167530701No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTTTCGTCCAGAA1960.1931757702391043No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGTGCTCCCGTA1850.1823342729297668No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTGTCGTCCAGTA1780.17543513827837023No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGCCCAGAG1550.15276655299521003No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGGCCAAAA1470.14488182767932822No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG1300.12812678638307937No Hit
CCTGTTCGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1060.10447261043543396No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCCGG56.450792E-43547.1802195
GTACCCG56.450792E-43547.1802195
ACTCCGG56.450792E-43547.1802195
TCAACGG56.450792E-43547.1802195
GTAACCG56.450792E-43547.1802195
CCGGCCG56.450792E-43547.1802195
GGAACCG650.03547.18195
TCTCCAG3000.03310.7014195
TAATCCG500.03192.4622195
TTCGCCG450.03153.049195
CCAACCG201.0440999E-92660.385195
TAAACCA201.0440999E-92660.385195
TCCACGG700.02026.9601195
CCAGCCG100.00257984061773.5901195
CTGGCCG100.00257984061773.5901195
TATCCAG100.00257984061773.5901195
ATCCCCA256.4646883E-9194.054729
GTTTGCT8400.0194.054724
CGATACC1800.0194.054727
TCGATAC1800.0194.054726
Produced by FastQC (version 0.11.5)