FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301495

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301495
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132097
Sequences flagged as poor quality0
Sequence length198-201
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA3529726.72051598446596No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA3064823.201132501116604No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA1720213.022248802016701No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA107728.154613655117073No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA64004.844924563010515No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGCGCA50713.8388456967228626No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA29552.236992513077511No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA26422.0000454211677785No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA23901.809276516499239No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA15681.1870065179375762No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTCATTGGGCGTAAAGCGCTCGTA14011.0605842676215207No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA13941.0552851313807277No Hit
TACGGAAGGTTCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA11010.8334784287304028No Hit
TACGTATGTCGCAAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA8560.6480086603026564No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA7590.5745777723945282No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCGCGCA6070.4595108140230285No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGAGCGCA5550.4201458019485681No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGGGCGCA3980.3012937462622164No Hit
TACGGAGGGTGCAAGCGTTACTCGGAATCACTGGGCGTAAAGGACGCGTA3600.2725270066693415No Hit
TACGTAGGTCCCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA3120.23619007244676263No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA3000.22710583889111788No Hit
TACGTATGTCGCGAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA2910.22029266372438436No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGTTTAAAGGGAGCGTA2730.20666631339091726No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATCATTGGGCGTAAAGGGAGCGCA2210.16730130131645685No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA1950.1476187952792266No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA1900.14383369796437467No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGCGCA1840.13929158118655233No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA1680.12717926977902602No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA1360.10295464696397345No Hit
TACGTATGTCACAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGTCTA1350.10219762750100306No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGATAG1500.0502.8139195
AGGCAGG350.0502.8139195
AAGGCAG35800.0492.2801195
AAGCGGG1550.0356.83566195
AAGGCGG7600.0344.03055195
GGATGCG100.002947981194.385857
AAGTAGG100.002947981194.385852
CGGAGGA100.002947981194.385853
GAGGATG100.002947981194.385855
ACGTCGG100.002947981194.385852
AGGATGC100.002947981194.385856
GTCGCGA400.0194.385858
GTCGCAA1050.0194.385858
TAAGTAG100.002947981194.385851
GGTCCGG7300.0194.385858
GATGCGA100.002947981194.385858
TCGCGAG400.0194.385859
ACGTAAG100.002947981194.385852
ATGCGAG100.002947981194.385859
CGTAGGG15050.0194.385853
Produced by FastQC (version 0.11.5)