FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301498

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301498
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151471
Sequences flagged as poor quality0
Sequence length198-201
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA2474716.337780829333667No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1674611.055581596477214No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC104826.920136527784196No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC94676.250041262023753No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG80965.344917508962112No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA68064.493269338685293No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG54803.61785424272633No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG53763.549194235200137No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTGTTATCCCAGGA46333.0586712968158922No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC43852.8949435865611237No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG39932.6361481735777805No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTATCGTCCAGTA36522.4110225719774743No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG30091.9865188715991842No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTGAGTTCCAGCA28001.8485386641667383No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGTGCTCCCGTA25181.6623644129899453No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTACACTCCCGTA25151.6603838358497665No Hit
CCTGTTCGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTATCGTCCAGTG20961.3837632286048154No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGTA20731.3685788038634457No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTGTCGTCCAGTA20671.3646176495830884No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTGATCGTCCAGAA16941.1183658918208768No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTTTCGTCCAGCA11770.7770464313300895No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG11700.7724250846696727No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTGCTGTCCAGTG8900.587571218252999No Hit
CCTGTTTGCTCCCCTAGCTTTCGCTCCTCAGCGTCAGTTATGGCCCAGAA8430.5565421763902001No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTGCAGCCCGGAC8190.5406975592687709No Hit
CCCGTTCGCTCCCCTGGCTTTCGAGCCTCAGCGTCAGTTGCAGTCCAGAA7140.47137735936251823No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGTGTCAGTTGCAGTCCAGAA6980.46081428128156543No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGGCCAAAA5620.3710281175934667No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGCGTCAGTTGTACCTTAGTA5160.34065926811072744No Hit
CCTGTTCGCTCCCCCGGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG5130.3386786909705488No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTATCGTCCAGTT4950.3267952281294769No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTAACGCTCCCGCA4460.294445801506559No Hit
CCTGTTCGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCACTCCGGAC3960.2614361825035816No Hit
CCTGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA3470.22908675588066363No Hit
CCTGTTCGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCACTCCAGAC3420.22578579398036588No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG3280.21654310065953217No Hit
CCTGTTCGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTATCATCCAGTG3170.20928098447887716No Hit
CCTGTTCGATACCCGCACCTTCGTGCTTAAGCGTCAGTAACGTTACGGTA2990.19739752163780525No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA2530.167028672155066No Hit
CCTGTTTGCTCCCCTCGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG2230.1472229007532795No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCCTCAGCGTCAGTTGTATCTTAGTG2140.14128116933274357No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAA2020.13335886077202896No Hit
CCTGTTTGATCCCCACGCTTTCGAGCCTCAACGTCAGTTATTGTCCAGTA1930.12741712935149302No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC1920.12675693697143348No Hit
CCTATTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCTTTGGCTAGCA1850.12213559031101662No Hit
CCTGTTTGCTACCCACGCTTTCGGGCATGAACGTCAGTGTTATCCCAGGA1790.11817443603065933No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG1590.10497058842946834No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGCCG8650.0399.39053195
TCTGCGG550.0399.39053195
TCGTCCG1100.0399.39053195
ATTTCCG550.0399.39053195
AATCCGG103.4092128E-4399.3905195
CCAGCCG1550.0399.3905195
TATCCGG103.4092128E-4399.3905195
ATTGCCG258.54925E-11399.3905195
GTAACCG5050.0399.3905195
GGAACCG258.54925E-11399.3905195
CAAACGG201.3551471E-8399.3905195
TAATCCG3250.0387.1016195
CCAACCG2800.0385.12656195
CAAGCCG4800.0382.7492195
GTCACCG2300.0382.02573195
GAAGCCG2500.0375.4271195
ATATCCG1600.0374.42862195
TCTCCGG9850.0366.95273195
TTCGCCG2500.0327.50024195
TATCCAG254.127105E-8319.51242195
Produced by FastQC (version 0.11.5)