FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences229553
Sequences flagged as poor quality0
Sequence length197-201
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTGTTATCCCAGGA5469323.825870278323524No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG3469915.115899160542446No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA228399.949336318845758No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG120515.249768027427217No Hit
CCTGTTCGATACCCGCACCTTCGTGCTTAAGCGTCAGTAACGTTACGGTA89083.880585311453128No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCATCAGCGTCAATAAATTGTTAGTA72953.1779153398125928No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG63752.7771364347231358No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG61202.6660509773342103No Hit
CCTGTTTGCTCCCCCGGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG54112.3571898428685314No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTCAGCGTCAGTATCTGTCCAGTA33401.455001677172592No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG32661.4227651130675705No Hit
CCCGTTCGCTCCCCTGGCTTTCGAGCCTCAGCGTCAGTTGCAGTCCAGAA28511.2419789765326528No Hit
CCTGTTTGCTACCCACGCTTTCGTGCATGAACGTCAGTGTTATCCCAGGA20720.9026237949406021No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC18520.8067853611148623No Hit
CCTGTTCGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTATCGTCCAGTG18150.7906670790623516No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG17910.7802119771904528No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA14570.6347118094731935No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTATCGTCCAGTG14330.6242567076012947No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCATCAGCGTCAATTAATTGTTAGTA14090.6138016057293958No Hit
CCTGTTTGCTCCCCACGCTTTCGTACATGAGCGTCAGTACATTCCCAAGG13750.5989902114108724No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGCGTCAGTTGTACCTTAGTA12160.5297251615095424No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTATCGTCCAGTA11950.520576947371631No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTGCAGCCCGGAC11650.5075080700317574No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGTGTCAGTTATGCCTTAGTA9960.4338867276838029No Hit
CCCGTTCGCTCCCCTGGCTTTCGTGCCTCAGCGTCAGTTTTCGTCCAGAA9250.40295705131276877No Hit
CCTGTTCGCTCCCCACGCTTTCGTCTCTCAGCGTCAATTGTCTGTTAGTC9250.40295705131276877No Hit
CCTGTTTGCTACCCACGCTTTCGCGCATGAACGTCAGTGTTATCCCAGGA9240.40252142206810626No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAGCGTCAGTGTTATCCCAGGA8560.3728986334310595No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG8470.36897797022909745No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGTGCTCCCGTA8090.35242405893192424No Hit
CCTGTTTGATCCCCACGCTTTCGAGCCTCAACGTCAGTTATTGTCCAGTA7970.3471965079959748No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTGAACGTCAGTGTTATCCCAGGA7870.3428402155493503No Hit
CCTGTTTGATACCCGCACCTTCGAGCCTCAGCGTCAGTTACGCTCCGGAT7020.30581172975304177No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTGAGTTCCAGCA6860.2988416618384426No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTACATTCCCAAGG6200.27009013169072066No Hit
CCTGTTTGCTACCCACGCTTTCGGGCATGAACGTCAGTGTTATCCCAGGA6070.2644269515101088No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAGGG5920.257892512840172No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCCTCAGCGTCAGTTGTATCTTAGTG5860.25527873737219725No Hit
CCTGTTTGCTCCCCTCGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG5660.24656615247894823No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCATCAGCGTCAATAACTTGTTAGTA5530.24090297229833632No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC5460.23785356758569917No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTTTCGTCCAGCA5330.23219038740508727No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTGTCGTCCAGTA5010.21825025157588882No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTACACTCCCGTA4880.2125870713952769No Hit
CCTGTTTGCTCCCCTAGCTTTCGTACTTCAGCGTCAGTTACCGTCCAGTG4840.2108445544166271No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCATGAACGTCAGTGTTATCCCAGGA4380.19080560916215428No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACCTTCCCAAGG4200.18296428275823012No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGGCCAAAA3840.16728162995038184No Hit
ACTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTGTTATCCCAGGA3360.1463714262065841No Hit
CCTGTTTGCTACCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG3140.13678758282401016No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAAGCCAGAG2900.12633248095211128No Hit
CCTGTTCGATACCCGCACCTTCGCGCTTAAGCGTCAGTAACGTTACGGTA2790.1215405592608243No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAGCGTCAGTTGTTGCCCAGAA2760.12023367152683694No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTGCTGTCCAGTG2480.10803605267628827No Hit
ACTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG2450.1067291649423009No Hit
CCTGTTCGATACCCGCACCTTCGAGCCTCAGCGTCAGTTACGCTCCGGAT2430.105857906452976No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCGG550.0261.64822195
CCAACCG2200.0261.64822195
GTACCCG400.0261.64822195
TAGCCCA950.0261.64822195
ACACCGG100.0012115373261.64822195
CTGGTCG100.0012115373261.64822195
TAACCGT100.0012115373261.64822195
GTCCCCG100.0012115373261.64822195
GTCTCCG151.1670285E-5261.64822195
TAATCCG600.0261.64822195
CTTGCCG151.1670285E-5261.64822195
CTGTCCG100.0012115373261.64822195
TGAACCG100.0012115373261.64822195
AATGCCG100.0012115373261.64822195
GGAACCG1150.0261.64822195
CAAACGG10200.0261.64822195
CAAATGG100.0012115373261.64822195
ATTTCCG100.0012115373261.64822195
CCGGCCG201.1224256E-7261.64822195
GTAACCG63100.0261.23358195
Produced by FastQC (version 0.11.5)