FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110905
Sequences flagged as poor quality0
Sequence length197-201
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG2622123.642757314818986No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA2191719.761958432893017No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC81457.3441233488120465No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG64075.777016365357738No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG54494.913214011992246No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTATCGTCCAGTA47584.290158243541771No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG45114.067445110680312No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG37953.421847527162887No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC28392.5598485190027502No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGTGCTCCCGTA24542.2127045669717327No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTGCTGTCCAGTG21791.9647446012352916No Hit
CCCGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA16151.4562012533249176No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTGTCGTCCAGTA15421.390379153329426No Hit
CCCGTTCGCTCCCCTGGCTTTCGAGCCTCAGCGTCAGTTGCAGTCCAGAA12571.1334024615662053No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG9340.8421622109012218No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTTTCGTCCAGCA8530.7691267300843064No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG7980.7195347369370182No Hit
CCTGTTCGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTATCGTCCAGTG6950.6266624588611874No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTACACTCCCGTA6900.6221540958477977No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC5780.5211667643478652No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGGCCAAAA5310.47878815202200087No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTAACGCTCCCGCA4650.419277760245255No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTGAGTTCCAGCA4300.3877192191515261No Hit
CCTGTTTGATCCCCACGCTTTCGAGCCTCAACGTCAGTTATTGTCCAGTA3920.3534556602497633No Hit
CCTGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA3550.3200937739506785No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG3430.30927370271854293No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA3180.2867318876515937No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTTTGGTCCAGCA2840.2560750191605428No Hit
CCTGTTTGCTCCCCTAGCTTTCGCTCCTCAGCGTCAGTTATGGCCCAGAA2830.25517334655786483No Hit
CCTGTTCGCTCCCCTAGCTTTCGCACTTCAGCGCCAGTTATCGTCCAGTG1950.17582615752220368No Hit
CCCGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1860.16771110409810197No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG1450.1307425273883053No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG1240.11180740273206799No Hit
CCTGTTCGATACCCGCACCTTCGCGCTTAAGCGTCAGTTGCGCTCCCGTC1130.10188900410261034No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACCG950.0606.1927195
CTGGCCG950.0606.1927195
ATTGCCG201.6752892E-9606.1927195
GTAACCG109.741412E-5606.1927195
ATATCCG2400.0606.1927195
AAGCCGT109.741412E-5606.1927195
TAATCCG350.0606.1926195
GGAACCG700.0606.1926195
TCTCCGG4600.0593.0146195
CCAACCG3950.0590.846195
CAAGCCG4900.0575.26447195
GAAGCCG800.0568.30566195
TTCGCCG1300.0559.5625195
GCAACCG600.0555.67664195
CATTCCG302.0008883E-11505.16058195
TCCACGG3100.0498.64233195
TCTACAG201.2766013E-6454.6445195
TCTCCAG11000.0416.0686195
CCACCCG153.2836798E-4404.12845195
CCGGCCG153.2836798E-4404.12845195
Produced by FastQC (version 0.11.5)