FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97439
Sequences flagged as poor quality0
Sequence length198-201
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA2224022.82453637660485No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGTA1265912.991717895298596No Hit
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGTA1182912.13990291361775No Hit
TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA87618.991266330729996No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA65936.766284547255205No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA65166.687260747749875No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA57385.8888124878128885No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA47654.890239021336426No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA43754.489988608257474No Hit
TACGTAGGGTGCAAGCGTTGTCCGGAATTACTGGGCGTAAAGAGCTCGTA32373.3220784285553013No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA22522.311189564753333No Hit
TACGTAGGGGGCGAGCGTTATCCGGATTCATTGGGCGTAAAGCGCGCGTA20282.08130214801055No Hit
TACGAAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA14361.4737425466189102No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGTA1660.1703629963361693No Hit
TACGGAGGCTCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA1320.13546937058056835No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGAGCGCA1110.11391742526093249No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGCGA100.0023144307210.7181195
ACGGCAG100.0023144307210.7181195
AAGGCAG38100.0210.44156195
AAGGCGA16450.0209.43713195
AAGGCGG34700.0207.98544195
GTGCAAG24400.0194.925069
ACAGAGG20650.0194.925062
TACAGAG20650.0194.925061
GGTCCCG6750.0194.925067
ACGTAGG59250.0194.925062
GTCCCGA6750.0194.925068
TACGTAG59250.0194.925061
AGGTCCC6750.0194.925066
GTAGGTC6750.0194.925064
TCCCGAG6750.0194.925069
GGGCGAG1850.0194.925069
GGTGCAA24400.0194.925068
TAGGTCC6750.0194.925065
GGTTGCA204.8591755E-7194.925057
TTGCAAG204.8591755E-7194.925059
Produced by FastQC (version 0.11.5)