FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87376
Sequences flagged as poor quality0
Sequence length198-201
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA1588318.177760483427942No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA1225114.021012635048526No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA1169513.384682292620397No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA1035011.845357993041567No Hit
TACGGAAGGTTCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA55736.378181651712141No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA40664.653451748763962No Hit
TACGTATGTCGCGAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA39834.558459989013001No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA33123.7905145577733017No Hit
TACGTATGTCGCAAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA29473.3727797106757005No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA14061.6091375206006227No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCGCGCA12521.4328877494964292No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGTGTA11291.2921168284197033No Hit
TACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA8831.0105749862662516No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGAGCGCA5810.6649423182567296No Hit
TACGGAAGGTCCTGGTGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA4850.5550723310748947No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA3750.4291796374290423No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATCATTGGGCGTAAAGGGAGCGCA3360.3845449551364219No Hit
TACGCAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA3240.37081120673869256No Hit
TACGCAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA3130.3582219373741073No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTCATTGGGCGTAAAGCGCTCGTA2970.3399102728438015No Hit
TACGCAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA2880.3296099615455045No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA2390.27353048892144294No Hit
TACGGAAGGTTCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA2270.2597967405237136No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA2060.23576268082768723No Hit
TACGTAGGGAGCGAGCGTTATCCGGATTTATTGGGTGTAAAGGGTGCGTA1870.2140175791979491No Hit
TACGCAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA1490.1705273759384728No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA1450.16594945980589634No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA1340.1533601904413111No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGAGCGCA1260.1442043581761582No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA1230.14077092107672587No Hit
TACGCATGTCGCGAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA1230.14077092107672587No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGTA1200.13733748397729353No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGCGTAAAGGGAGCGCA1060.12131477751327596No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGGGTGCGTA1060.12131477751327596No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA1050.12017029848013185No Hit
TACGGAGGGTGCAAGCGTTACTCGGAATCACTGGGCGTAAAGGACGCGTA1040.11902581944698773No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA900.10300311298297016No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGG107.9555175E-4300.92566195
AAGGCAT900.0300.92566195
AAGATAG7100.0298.8065195
AAGGCAG29050.0296.26416195
AAGGCGG16550.0261.8326195
AAGCGGG500.0240.74054195
AGGCAGG402.910383E-11225.69424195
GTATGTC7200.0194.645694
ACGCATG256.340997E-9194.645692
GGCGCGA4850.0194.645698
GGTCCGG16300.0194.645698
GGTCCCG15250.0194.645697
TACGGAG450.0194.645691
GGTTCGG5650.0194.645698
GCGCGAG4850.0194.645699
GTCCCGA15250.0194.645698
TACGCAT256.340997E-9194.645691
TACGTAT7200.0194.645691
TACGTAG49500.0194.645691
GCATGTC256.340997E-9194.645694
Produced by FastQC (version 0.11.5)