FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87376
Sequences flagged as poor quality0
Sequence length197-201
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC1647818.85872550814869No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA1322215.132301776231461No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1047811.991851309284014No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTATCGTCCAGTA71798.216214978941586No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG42194.828557040835012No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC36104.1315693096502475No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTGCTGTCCAGTG29153.3361563816150888No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG16651.905557590184948No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTTTCGTCCAGCA14571.6675059512909725No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG14221.6274491851309285No Hit
CCTGTTCGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTATCGTCCAGTG13891.5896813770371727No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTACACTCCCGTA10761.2314594396630654No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGTA10411.1914026735030214No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC9981.1421900750778247No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGTGCTCCCGTA9821.1238784105475188No Hit
CCTGTTCGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCACTCCGGAC7940.9087163523164256No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTGTCGTCCAGTA7100.8125801135323201No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTGATCGTCCAGAA5810.6649423182567296No Hit
CCCGTTCGCTCCCCTGGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAA5670.648919611792712No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTAACGCTCCCGCA4630.5298937923457242No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG4250.4864035890862479No Hit
CCTGTTCGATACCCGCACCTTCGCGCTTCAGCGTCAGTTGCGCTCCCGTC3440.39370078740157477No Hit
CCTGTTCGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTATCATCCAGTG3380.3868339132027101No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG3320.37996703900384543No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG3220.3685222486724043No Hit
CCCGTTCGCTCCCCTGGCTTTCGAGCCTCAGCGTCAGTTGCAGTCCAGAA3090.3536440212415308No Hit
CCTGTTTGCTCCCCTAGCTTTCGCTCCTCAGCGTCAGTTATGGCCCAGAA2660.304431422816334No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTATCGTCCAGTT2330.26666361472257827No Hit
CCTGTTTGCTCCCCACGCCTTCGCGCCTCAACGTCAGTTATCGTCCAGTA2170.24835195019227246No Hit
CCTGTTTGCTCCCCTCGCTTTCGCACTTCAGCGTCAGTTGCTGTCCAGTG2030.2323292437282549No Hit
CCCGTTCGCTTCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA2010.23004028566196666No Hit
CCTGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA1800.20600622596594031No Hit
CCTGTTTGATCCCCACGCTTTCGAGCCTCAACGTCAGTTATTGTCCAGTA1780.20371726789965208No Hit
CCCGTTCGCTCCCCTCGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA1600.18311664530305805No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC1380.15793810657388757No Hit
CCCGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1250.1430598791430141No Hit
CCTATTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCTTTGGCTAGCA1210.13848196301043764No Hit
CCTGTTCGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTATCGTCCAGTA1170.1339040468778612No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGTGTCAGTTTTCGTCCAGAA1140.13047060977842886No Hit
CCTGTTCGCTACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC1020.1167368613806995No Hit
CCTGTTTGCTCCCCTCGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG1020.1167368613806995No Hit
CCGGTTCGCTCCCCACGCTTTCGTGCCTTAGCGTCAGAAATGGCCCAGTA950.10872550814869071No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTATCGTCCAGTG930.10643655008240249No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG930.10643655008240249No Hit
CCTGTTTGCTCCCCTAACTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG920.10529207104925838No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTGAGTTCCAGCA900.10300311298297016No Hit
CCTGTTCGATACCCGCACCTTCGCGCTTAAGCGTCAGTTGCGCTCCCGTC890.10185863394982604No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCGG750.0346.47504195
TATCCGG105.2136206E-4346.47504195
ATTGCCG450.0346.47504195
TCGTCCG800.0346.47504195
AAGCCGT153.7874852E-6346.47504195
CTGGCCG4350.0342.49258195
TCTCCGG9650.0339.2942195
ATATCCG2500.0332.61603195
CAAGCCG9150.0331.32858195
TCCACGG1400.0321.72684195
CCAACCG1150.0316.34677195
CCAGCCG650.0293.1712195
GAAGCCG1300.0293.1712195
TAATCCG258.3578925E-8277.18002195
GCAACCG201.1942837E-5259.8563195
TTCGCCG700.0247.48218195
TCTCCAG18050.0244.73999195
CGAACCG150.0017558052230.98335195
ATTTCCG150.0017558052230.98335195
TGAGACC100.0029385067194.557547
Produced by FastQC (version 0.11.5)