FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301552

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301552
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences248932
Sequences flagged as poor quality0
Sequence length197-201
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA2811411.29384731573281No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG2725510.94877315893497No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG2557610.274291774460496No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC212978.5553484485723No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG163786.579306798643807No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTGTTATCCCAGGA141315.676650651583565No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA102314.1099577394629865No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG98143.9424421127054776No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG93393.7516269503318176No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC83853.368389761059245No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGGCCAAAA53962.1676602445647806No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG38801.5586585894943197No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTGCTGTCCAGTG32051.2875002008580656No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTACACTCCCGTA31541.2670126781611042No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG31261.2557646264843412No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTGAGTTCCAGCA19590.7869619012421062No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC19100.7672778108077708No Hit
CCTGTTCGCTCCCCCGGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG18570.7459868558481835No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG15250.6126171002522778No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTGTCGTCCAGTA13560.544727074060386No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGTA13180.5294618610704931No Hit
CCTGTTTGCTCCCCTCGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG12040.48366622210081467No Hit
CCCGTTCGCTCCCCTGGCTTTCGTGCCTCAGCGTCAGTTTTCGTCCAGAA11100.44590490575739555No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGTGCTCCCGTA10920.43867401539376216No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGACCAGAG10540.42340880240386936No Hit
CCTGTTTGCTCCCCTAGCTTTCGTACTTCAGCGTCAGTTACCGTCCAGTG10450.41979335722205263No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTTTCGTCCAGCA10330.414972763646297No Hit
CCTGTTTGATCCCCACGCTTTCGAGCCTCAACGTCAGTTATTGTCCAGTA10170.4085453055452895No Hit
CCTGTTCGATACCCACGCTTTCGAGCATCAGCGTCAGTTGCGCTACAGTA8960.3599376536564202No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTCAGCGTCAGTATCTGTCCAGTA7520.3020905307473527No Hit
CCTGTTTGATACCCGCACCTTCGTGCTTAAGCGTCAGTTGCGCTCCCGTC7400.29726993717159705No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTATCGTCCAGTA6710.2695515241110022No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGACCAGAG6700.26914980797968924No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCATCAGCGTCAATTAATTGTTAGTA5890.23661080134333873No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG5880.2362090852120258No Hit
CCTGTTTGATACCCGCACCTTCGCGCTTAAGCGTCAGTTGCGCTCCCGTC5870.23580736908071281No Hit
CCTGTTTGCTACCCACGCTTTCGGGCATGAACGTCAGTGTTATCCCAGGA5740.23058505937364418No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGTGTCAGTTGCAGTCCAGAA5310.21331126572718653No Hit
CCTGTTTGCTACCCACGCTTTCGTGCATGAACGTCAGTGTTATCCCAGGA5220.2096958205453698No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCCTCAGCGTCAGTTGTATCTTAGTG4540.18237912361608793No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG4410.17715681390901933No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAAGCCAGAG4100.1647036138383173No Hit
CCTGTTTGCTACCCACGCTTTCGTGCTTTAGCGTCAGTATCTGTCCAGTA3990.1602847363938746No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTTTGGTCCAGCA3630.14582295566660775No Hit
CCTGTTCGATACCCGCACCTTCGTGCTTAAGCGTCAGTTGCGCTCCCGTC3610.1450195234039818No Hit
CCTGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA3590.1442160911413559No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAGCGTCAGTTGTTGCCCAGAA3580.1438143750100429No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTAACGCTCCCGCA3560.14301094274741696No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC3420.1373869169090354No Hit
CCTGTTTGCTCCCCACGCTTTCGTACATGAGCGTCAGTACATTCCCAAGG3200.12854916202015007No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG3140.12613886523227227No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTAACACTCCCGCA3110.12493371683833336No Hit
CCTGTTTGCTCCCCAAACTGTCGTCCCTCATCGTCAAGTATTCTATAGTT2650.10645477479793677No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGCGTCAGTTGTACCTTAGTA2640.10605305866662382No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCATGAACGTCAGTGTTATCCCAGGA2530.10163418122218115No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGCGG800.0351.7897195
AAACCGG202.561137E-8351.7897195
GTCCCCG104.990987E-4351.7897195
ATAGCCG202.561137E-8351.7897195
ATTGCCG1300.0351.7897195
GATCCGG400.0351.7897195
CAAATCG104.990987E-4351.7897195
GTACCCG153.5781304E-6351.78967195
GTAACCG16900.0351.78967195
CAAACGG301.8189894E-12351.78967195
CTGGCCG30850.0350.64938195
GGAACCG3650.0346.97064195
GTCACCG10600.0346.81152195
ATATCCG3450.0346.69128195
TAATCCG2500.0344.7539195
CCAACCG2750.0326.205195
AAGGCCG2000.0325.40546195
CCAGCCG2300.0313.5517195
TAAACCA6050.0302.3647195
AATCCGG353.6379788E-12301.53403195
Produced by FastQC (version 0.11.5)