FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126889
Sequences flagged as poor quality0
Sequence length197-201
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA3596528.34367045212745No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA2955223.289646856701527No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA1651813.017676867183129No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA1473111.609359361331558No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA60584.7742515111633No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA36332.863132344017212No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA23831.8780193712614963No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA20031.578545027543759No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCGCGCA18891.4887027244284374No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA18731.4760932783771643No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA17711.395708059800298No Hit
TACGGAGGGTGCAAGCGTTACTCGGAATCACTGGGCGTAAAGGACGCGTA10660.8401043431660742No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTCATTGGGCGTAAAGCGCTCGTA6030.4752184980573572No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA3090.2435199268652129No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA2880.22697002892291687No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA2780.21908912514087114No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTCTAAAGGGAGCGTA1830.14422053921143677No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA1770.13949199694220932No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGCGCA1310.103239839544799No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGATAG101.6371945E-4509.97104195
CAGGCAG101.6371945E-4509.97104195
AAGGCAG24750.0506.88028195
AAGCGGG5900.0497.0057195
AAGGCGG8550.0432.4316195
AGGCGAG155.5174384E-4339.98068195
GTATGTC204.9332994E-7194.379994
GAGGATG100.002948111194.379995
GAGGATA100.002948111194.379995
GATACGA100.002948111194.379998
AGGATGC100.002948111194.379996
AGGATAC100.002948111194.379996
GTCGCAA100.002948111194.379998
GTTCCGA100.002948111194.379998
GGTCCGG6850.0194.379998
TCGGTCC100.002948111194.379995
TACGGAA22100.0194.379991
GGTTCCG100.002948111194.379997
ATGCGAG100.002948111194.379999
GGATACG100.002948111194.379997
Produced by FastQC (version 0.11.5)