FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301554

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301554
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126889
Sequences flagged as poor quality0
Sequence length197-201
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA3430327.033864243551452No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG2643620.833957238216076No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1472711.60620699981874No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC126589.97564800731348No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC41663.2831845156002486No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG35042.7614686852288224No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG20071.5816973890565769No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG18121.4280197653066853No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTGTCGTCCAGTA17981.4169865000118214No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG17011.340541733325978No Hit
CCTGTTCGATACCCACACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC14681.1569166752043125No Hit
CCCGTTCGCTCCCCTGGCTTTCGTGCCTCAGCGTCAGTTTTCGTCCAGAA13471.0615577394415592No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGGCCAAAA11300.890542127371167No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG10890.8582304218647795No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGTGCTCCCGTA10450.8235544452237783No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGACCAGAG10300.8117330895507097No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTGAGTTCCAGCA8550.6738172733649095No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG6790.5351133668009047No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTGATCGTCCAGAA6680.5264443726406544No Hit
CCTGTTTGCTCCCCTAGCTTTCGCTCCTCAGCGTCAGTTATGGCCCAGAA5450.42950925612149204No Hit
CCTGTTTGATACCCGCACCTTCGTGCTTAAGCGTCAGTTGCGCTCCCGTC5320.41926408120483255No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGACCAGAG5100.401926092884332No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG3940.31050760901260155No Hit
CCTGTTTGATACCCGCACCTTCGCGCTTAAGCGTCAGTTGCGCTCCCGTC3810.3002624340959421No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTTTCGTCCAGCA3060.24115565573059916No Hit
CCTGTTTGATCCCCACGCTTTCGAGCCTCAACGTCAGTTATTGTCCAGTA2800.2206653058972803No Hit
CCTGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA2510.1978106849293477No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC1720.13555154505118647No Hit
CCTGTTCGATACCCGCACCTTCGTGCTTCAGCGTCAGTTGCGCTCCCGTC1570.12373018937811787No Hit
CCTGTTCGCTCCCCATGCTTTCGTTTCTCAGCGTCAGTTACAGCCCAGAG1440.11348501446145844No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCGG400.0656.3569195
GTCACCG1650.0656.3569195
GGCACCG107.6773846E-5656.3569195
TACGCCA107.6773846E-5656.3569195
TATCCGG152.944962E-7656.3569195
TATCCAG107.6773846E-5656.3569195
GATCCGG107.6773846E-5656.3569195
AAAACCG152.944962E-7656.3569195
GTAACCG201.1259544E-9656.3569195
GGAACCG1850.0656.3569195
GGAACCA201.1259544E-9656.3569195
AAACCGG253.6379788E-12656.3568195
TCTGCGG253.6379788E-12656.3568195
TAATCCG650.0656.3568195
TCTCCAG20650.0651.5892195
GAAGCCG1700.0637.05225195
TAAACCA950.0621.81177195
TTCGCCG2450.0589.38165195
CCAACCG2400.0587.9863195
TAACCGG253.434252E-9525.08545195
Produced by FastQC (version 0.11.5)