FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121039
Sequences flagged as poor quality0
Sequence length197-201
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG5579046.09258172985567No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG2054616.97469410685812No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG1488012.293558274605704No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA81586.73997637125224No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGACCAGAG21691.7919844017217592No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG16471.3607184461206718No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG13811.1409545683622635No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG12561.0376820694156428No Hit
CCTGTTCGCTACCCACGCTTTCGAGCCTCAGCGTCAGTGACAGACCAGAG8200.6774675930898305No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGACCAGAG8080.667553433190955No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG7570.6254182536207338No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG6410.52958137459827No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG6260.5171886747246756No Hit
CCTGTTCGCTCCCCATGCTTTCGTTTCTCAGCGTCAGTTACAGCCCAGAG5610.4634869752724329No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC4840.39987111592131463No Hit
CCCGTTCGCTCCCCTGGCTTTCGTGCCTCAGCGTCAGTTTTCGTCCAGAA3200.26437759730334853No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC2860.23628747758986773No Hit
GCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG2680.22141623774155436No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGGCCAAAA1880.15532183841571726No Hit
CCTGTTCGCTCCCCTCGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG1460.12062227876965276No Hit
CCTGTTCGCTCCCCTCGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1410.11649137881178794No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACTAGAG1400.11566519882021498No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACCGG350.03530.88195
TGCACGG56.520271E-43530.8796195
AATCCGG56.520271E-43530.8796195
TAAACCA200.03530.8796195
TTACCCG56.520271E-43530.8796195
GGACCCG56.520271E-43530.8796195
GAAGCCG200.03530.8796195
TCAATTG56.520271E-43530.8796195
ATAACCG56.520271E-43530.8796195
TCTGCGG56.520271E-43530.8796195
TAACCGG153.4015102E-103530.8796195
TAATCCG153.4015102E-103530.8796195
AATACGG56.520271E-43530.8796195
TCTCCAG1000.03530.8796195
CAGGCCG56.520271E-43530.8796195
ATCGCCG56.520271E-43530.8796195
CAAACCA56.520271E-43530.8796195
GGAACCG1550.03530.8794195
GTCACCG850.03115.482195
AAAACCG400.03089.5198195
Produced by FastQC (version 0.11.5)