FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301574

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301574
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117818
Sequences flagged as poor quality0
Sequence length197-201
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA1937416.444006858035277No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG91247.744147753314435No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG79266.727325196489501No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC78986.7035597277156285No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG69355.886197355242833No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG65335.54499312498939No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG59765.0722300497377315No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC57464.877013699095214No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC49554.205639206233343No Hit
CCTGTTCGCTCCCCCGGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG39753.373847799147838No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG24722.0981513860360894No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGTA23602.0030895109406033No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG23111.9614999405863283No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA19471.6525488465259979No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGGCCAAAA19171.6270858442682783No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTGATCGTCCAGAA16421.3936749902391825No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG15821.3427489857237433No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTTTCGTCCAGCA13861.1763907043066424No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTACACTCCCGTA10950.9293995824067629No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTGTCGTCCAGTA9650.819059905956645No Hit
CCTGTTTGCTCCCCCGGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG9470.8037821046020133No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGTGCTCCCGTA7940.6739207930876436No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTATCGTCCAGTA7900.6705257261199477No Hit
CCCGTTCGCTCCCCTGGCTTTCGTGCCTCAGCGTCAGTTTTCGTCCAGAA7040.5975317863144851No Hit
CCTGTTTGCTCCCCTCGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG4100.3479943641888336No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTGAGTTCCAGCA3890.33017026260842997No Hit
CCTGTTTGCTCCCCTAGCTTTCGTACTTCAGCGTCAGTTACCGTCCAGTG3670.3114973942861023No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGACCAGAG2990.25378125583527134No Hit
CCTGTTCGCTCCCCACGCTTTCGTTCCTCAGCGTCAGTAACGGCCCAGAG2910.24699112189987948No Hit
CCTGTTCGATACCCGCACCTTCGTGCTTCAGCGTCAGTTGCGCTCCCGTC2890.24529358841603152No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGACCAGAG2540.21558675244869205No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAA2520.21388921896484409No Hit
CCTGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA2520.21388921896484409No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAAGCCAGAG2500.21219168548099612No Hit
CCTGTTCGATACCCGCACCTTCGCGCTTCAGCGTCAGTTGCGCTCCCGTC2480.21049415199714816No Hit
CCTGTTCGATACCCGCACCTTCGTGCTTAAGCGTCAGTTGCGCTCCCGTC2450.2079478517713762No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTAACGCTCCCGCA2390.20285525131983226No Hit
CCTGTTTGCTCCCCTAGCTTTCGCTCCTCAGCGTCAGTTATGGCCCAGAA2350.19946018435213633No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTATCGTCCAGTT2040.17314841535249284No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAGCGTCAGTTGTTGCCCAGAA2010.17060211512672085No Hit
CCTGTTTGATACCCGCACCTTCGTGCTTAAGCGTCAGTTGCGCTCCCGTC1980.16805581490094892No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTGTCATCCCAGGA1760.14938294657862128No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG1650.14004651241745744No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAAGCCAGAG1560.1324076117401416No Hit
CCTGTTCGCTCCCCCGGCTTTCGTACTTCAGCGTCAGTTACCGTCCAGTG1540.1307100782562936No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG1500.12731501128859765No Hit
CCCGTTCGCTCCCCTGGCTTTCGAGCCTCAGCGTCAGTTGCAGTCCAGAA1500.12731501128859765No Hit
CCTGTTCGATACCCGCACCTTCGCGCTTAAGCGTCAGTTGCGCTCCCGTC1430.12137364409512978No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC1430.12137364409512978No Hit
CCTGTTTGATACCCGCACCTTCGCGCTTAAGCGTCAGTTGCGCTCCCGTC1380.11712981038550985No Hit
CCTGTTTGATCCCCACGCTTTCGAGCCTCAACGTCAGTTATTGTCCAGTA1320.11203720993396594No Hit
CCTATTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCTTTGGCTAGCA1210.10270077577280211No Hit
CCTGTTCGCTCCCCACGCTTTCGTTCCTCAGCGTCAGTAACGGCCCAGTA1210.10270077577280211No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACCG153.5048724E-6353.40094195
TATCCAG104.91713E-4353.4009195
GATCCGG202.4932888E-8353.4009195
AAAACCG104.91713E-4353.4009195
AAGTCCA104.91713E-4353.4009195
CCAACCG6700.0350.76358195
CTGGCCG10350.0349.9864195
GTCACCG3050.0347.60745195
GAAGCCG1500.0341.6209195
GGAACCG1500.0341.6209195
CCAGCCG2350.0338.36258195
AAGGCCG5600.0328.158195
GCAACCG1200.0323.95087195
ATTGCCG550.0321.27356195
CCACCCG400.0309.2258195
TAATCCG600.0294.50076195
ATTTCCG305.311449E-10294.50076195
CAAGCCG1300.0244.66217195
TAAACCA2600.0237.866195
CAAACGG150.0016560223235.60062195
Produced by FastQC (version 0.11.5)