FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301609

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301609
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123894
Sequences flagged as poor quality0
Sequence length198-201
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA4529036.55544255573312No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA1880815.180719001727283No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA1580312.755258527450886No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA118199.539606437761313No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA42153.4021017966971767No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGCGCA41873.3795018322114068No Hit
TACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA36302.929923967262337No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA26142.109868113064394No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA25412.050946777083636No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA20381.6449545579285518No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA18941.5287261691445913No Hit
TACGTATGTCGCAAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA10660.8604129336368185No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA6300.5084992009298271No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA5250.42374933410818927No Hit
TACGTATGTCGCGAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA4430.357563723828434No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCTTGTA3240.2615138747639111No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA3130.25263531728735855No Hit
TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA2550.20582110513826338No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGAGCTTGTA2390.1929068397178233No Hit
TACAGAGGATGCAAGCGTTATCCGGAATGATTGGGCGTAAAGCGTCTGTA2240.18079971588616076No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGAGCGCA2120.17111401682083072No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA1860.15012833551261562No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTCATTGGGCGTAAAGCGCTCGTA1360.10977125607374044No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGCGCA1360.10977125607374044No Hit
TACGTAGGTCCCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA1250.10089269859718793No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGATAG1400.0467.53302195
AAGCCGG151.1446991E-6467.533195
AAGGCGG11400.0447.0272195
AAGGCAG17200.0443.0691195
AAGCGGG1100.0425.03195
AGGCAGG400.0409.0914195
GAAGGCA33400.0227.4674195
GGATGCA351.8189894E-12194.41227
GTCGCAA950.0194.41228
GATGCAA351.8189894E-12194.41228
CGTAGGG31850.0194.41223
TCGCAAG950.0194.41229
GTAGGTC66950.0194.41224
GTAGGGT5150.0194.41224
GTAGGGC24100.0194.41224
GTGCGAG9050.0194.41229
GGTGCGA9050.0194.41228
TGTCGCA950.0194.41227
GTATGTC1350.0194.412194
GGGCAAG2850.0194.412199
Produced by FastQC (version 0.11.5)