FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301615

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301615
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110516
Sequences flagged as poor quality0
Sequence length198-201
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA4321739.10474501429657No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA1977917.896956096854755No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA90058.14814144558254No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA88848.038655036374823No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA82597.47312606319447No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA75796.857830540374245No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA26162.3670780701436898No Hit
TACGGAGGGTGCAAGCGTTACTCGGAATCACTGGGCGTAAAGGACGCGTA20991.8992725035289006No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA14201.2848818270657643No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA3970.35922400376416086No Hit
TACGGGGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA3820.3456513084078323No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGGGAGCGCA3090.279597524340367No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTCCGTA3000.2714539071265699No Hit
TACGTATGTCGCGAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA2910.2633102899127728No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA2290.20720981577328168No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCTTGTA1730.156538419776322No Hit
TACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA1570.1420608780629049No Hit
TACGTAGGTCCCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA1530.13844149263455066No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTCATTGGGCGTAAAGCGCTCGTA1410.12758333634948785No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGTGTA1360.12305910456404502No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGAGCTTGTA1250.11310579463607079No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCGCGCA1200.10858156285062795No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGATAG157.326944E-7522.5803195
CAGGCAG101.5207964E-4522.5803195
AAGGCAG22100.0521.398195
AAGCGGG1600.0489.91904195
AAGGCGG4200.0472.81073195
GAGGGCA155.125355E-4348.38687195
GTGGCAA21100.0194.37158
CTTTCCC100.0029479838194.371496
GTATGTC308.367351E-11194.371494
AGGGGGC500.0194.371496
GGATGCG100.0029479838194.371497
TCCTTCT153.8162107E-5194.371491
GAGGATG100.0029479838194.371495
ACGTCGG100.0029479838194.371492
AGGATGC153.8162107E-5194.371496
GTCGCGA204.931517E-7194.371498
TTTCCCT100.0029479838194.371497
TGTCCCG100.0029479838194.371497
GGTCCGG9100.0194.371498
TACGGGG500.0194.371491
Produced by FastQC (version 0.11.5)