FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301627

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301627
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96517
Sequences flagged as poor quality0
Sequence length198-201
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA1404614.5528766952972No Hit
TACGGAAGGTTCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA1061911.002206865111845No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA1048710.865443393391839No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA87659.081301739589916No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA84868.792233492545355No Hit
TACGTATGTCGCGAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA61946.417522301770672No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA50485.230166706383332No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA41264.274894578157216No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGTGTA33473.467782877627775No Hit
TACGTATGTCGCAAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA28072.908295947864107No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATCATTGGGCGTAAAGGGAGCGCA26162.7104033486328833No Hit
TACGTATGTCACAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGTCTA20622.1364112021716384No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA15391.5945377498264555No Hit
TACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA12151.2588455919682544No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGCGCA10001.0360869069697565No Hit
TACGTAGGGGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGTGCGTA6770.7014308360185253No Hit
TACGGAAGGTCCTGGTGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA5880.6092191012982169No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGAGTGCGTA5840.6050747536703378No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA4870.5045743236942715No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA4450.4610586736015417No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCGCGCA4190.43412041402032797No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA3950.40925432825305386No Hit
TACGTAGGGGGCTAGCGTTATCCGGATTTACTGGGCGTAAAGGGTGCGTA3550.3678108519742636No Hit
TACGCAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA3120.32325911497456405No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGAGCGCA3100.3211869411606246No Hit
TACGCAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA3000.310826072090927No Hit
TACGGAGGGTGCAAGCGTTACTCGGAATCACTGGGCGTAAAGGACGCGTA2570.26627433509122744No Hit
TACGTATGTCGCGAGCGTTATCCGGAATTATTGGGTATAAAGGGCATCTA2300.23829998860304402No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGGGCGCA1840.19063999088243522No Hit
TACGCATGTCGCGAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA1830.18960390397546545No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGGGTGCGTA1700.17613477418485862No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA1580.16370173130122154No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA1510.15644912295243327No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA1480.15334086223152396No Hit
TACGCAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA1470.15230477532455422No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA1460.15126868841758445No Hit
TACGCAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA1340.1388356455339474No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA1340.1388356455339474No Hit
TACGTAGGGAGCGAGCGTTATCCGGATTTATTGGGTGTAAAGGGTGCGTA1290.1336552109990986No Hit
TACGCATGTCGCAAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA970.10050042997606638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGATAG10500.0257.8085195
AAGGCAG44750.0257.01602195
AAGGCGG19400.0196.28444195
ATGTCGC10550.0194.751316
ATGTCAC1750.0194.751316
TGGCGAG3500.0194.751319
ATGGGGC153.785138E-5194.75136
GTCACAA1650.0194.75138
ACGCATG500.0194.75132
GTCGCGA7700.0194.75138
GGCGCGA10600.0194.75138
GTCGCAA2850.0194.75138
GGTCCCG7750.0194.75137
TATGTCA1650.0194.75135
GGTCCAG13750.0194.75138
TACGGAG500.0194.75131
GATGCGA256.326445E-9194.75138
TCGCGAG7700.0194.75139
ACGTAGT153.785138E-5194.75132
TGTCACA1650.0194.75137
Produced by FastQC (version 0.11.5)