FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301633

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301633
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences214176
Sequences flagged as poor quality0
Sequence length198-201
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA4919622.96989391901987No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA4557321.27829448677723No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA2313410.80139698192141No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA155007.237038697146272No Hit
TACGTATGTCACAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGTCTA83713.9084678021813835No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGCGCA76973.59377334528612No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA64232.9989354549529357No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA62972.9401053339309726No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA60032.802835051546392No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA37911.7700395936052593No Hit
TACGTATGTCGCAAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA35251.6458426714477812No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCGCGCA31301.4614149111011503No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA30721.4343343792021517No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA26651.2443037501867622No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGTGTA24531.1453197370386972No Hit
TACGTATGTCGCGAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA15910.7428470043328851No Hit
TACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA15460.7218362468250411No Hit
TACGGAGGGTGCAAGCGTTACTCGGAATCACTGGGCGTAAAGGACGCGTA15450.7213693411026446No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTCATTGGGCGTAAAGCGCTCGTA6980.32590019423278055No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA6560.3062901538921261No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA6550.30582324816972956No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA6440.30068728522336774No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGAGTGCGTA6290.29368369938741967No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA6190.28901464216345435No Hit
TACGCAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA5370.2507283729269386No Hit
TACGCAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA5130.2395226355894218No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA5090.23765501269983566No Hit
TACGGAAGGTCCTGGTGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA4630.2161773494695951No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGGGCGCA4540.2119751979680263No Hit
TACGGAAGGTTCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA4380.20450470640968177No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGAGCGCA3500.1634170028387868No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGGGTGCGTA2970.1386709995517705No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTCCGTA2450.11439190198715077No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATCATTGGGCGTAAAGGGAGCGCA2420.11299118481996116No Hit
TACGTAGGTCCCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA2400.11205737337516809No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGATAG5700.0440.62912195
AAGATAA208.309144E-9440.6291195
AAGCCGG102.5408182E-4440.6291195
AAGGCAG54250.0432.913195
AAGCGGG3700.0369.17575195
AAGGCGG18450.0364.20563195
GTAGGCA200.00202576220.31454195
AAGCTAG200.00202576220.31454195
AGGCAGG1000.0198.2831195
GAGGTCC100.0029467724194.440585
TCCCAAG100.0029467724194.440589
GTCACAA8050.0194.440588
GAGGATG1050.0194.440585
ACAGAGG100.0029467724194.440582
ACGCATG100.0029467724194.440582
AGGATGC1050.0194.440586
TGTCCCG100.0029467724194.440587
GGCGCAA1950.0194.440588
TGCCGAG100.0029467724194.440589
GGTCCCA100.0029467724194.440587
Produced by FastQC (version 0.11.5)