FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106850
Sequences flagged as poor quality0
Sequence length198-201
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA1374812.866635470285448No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA1309912.25924192793636No Hit
TACGTATGTCGCAAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA1293212.10294805802527No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA1081510.121665886757135No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA88728.303228825456246No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGAGCGCA85387.990641085634066No Hit
TACGGAAGGTTCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA63785.969115582592419No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGTGTA54805.128685072531587No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA43014.02526906878802No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATCATTGGGCGTAAAGGGAGCGCA34103.191389798783341No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA24172.2620496022461394No Hit
TACGTATGTCGCGAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA11181.046326626111371No Hit
TACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA10891.0191857744501638No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA9910.9274684136640149No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCGCGCA9330.8731867103416004No Hit
TACGGAGGGTGCAAGCGTTACTCGGAATCACTGGGCGTAAAGGACGCGTA9240.8647636874122603No Hit
TACGGAAGGTCCTGGTGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA6630.6204960224613945No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA4910.45952269536733736No Hit
TACGTATGTCACAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGTCTA3530.33036967711745435No Hit
TACATATGGGGCGAGCGTTATCCGGATTTATTGGGTGTAAAGGGTGCGTA3270.30603649976602715No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA2960.27702386523163314No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA2580.24145999064108564No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA2210.20683200748713151No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGCGCA2050.1918577445016378No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA1990.1862423958820777No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGTGTA1910.17875526438933084No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA1840.17220402433317736No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGAGCGCA1500.14038371548900327No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA1440.13476836686944316No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTCATTGGGCGTAAAGCGCTCGTA1360.1272812353766963No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA1220.11417875526438934No Hit
TACGTAGGGAGCGAGCGTTATCCGGATTTATTGGGTGTAAAGGGTGCGTA1170.10949929808142256No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGCTTAAAGGGAGCGTA1070.100140383715489No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA1070.100140383715489No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGCAG100.0010466495274.64816195
AAGATAG16600.0271.33914195
AAGGCAT2700.0269.56213195
AAGGCAG41200.0266.98203195
AGGCAGG800.0257.48267195
AGATAGT203.0239526E-5205.98613195
GTATGGA153.7895923E-5194.708484
GGTCCCG5900.0194.708487
GGTCCAG19850.0194.708488
TGGAGCA153.7895923E-5194.708487
ATGGAGC153.7895923E-5194.708486
GAGCAAG153.7895923E-5194.708489
GTCCCGA5900.0194.708488
CGTATGG153.7895923E-5194.708483
GTCCAGG19900.0194.708489
AGGTCCG14100.0194.708487
AGGTCCC5950.0194.708486
AGGTCCA19850.0194.708487
GTAGGTC5950.0194.708484
GGGGCAA2400.0194.708488
Produced by FastQC (version 0.11.5)