FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301660

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301660
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105131
Sequences flagged as poor quality0
Sequence length197-201
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG2518623.9567777344456No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA1415613.465105439879768No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTGTTATCCCAGGA81957.795036668537349No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG69126.5746544786980055No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG57575.4760251495752925No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG55105.24108017616117No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC33933.227402003215037No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG29322.7889014657902997No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG23752.2590862828280907No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTGAGTTCCAGCA19971.8995348660242934No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG16041.5257155358552663No Hit
CCTGTTTGATCCCCACGCTTTCGAGCCTCAACGTCAGTTATTGTCCAGTA15511.4753022419647868No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA14341.3640125177159925No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC13491.2831610086463556No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGCGTCAGTTGTACCTTAGTA13461.2803074259733096No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGGCCAAAA13301.265088318383731No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTGTCGTCCAGTA12161.1566521768079823No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTGCTGTCCAGTG10991.045362452559188No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGACCAGAG9820.9340727283103936No Hit
CCTGTTCGATACCCGCACCTTCGTGCTTAAGCGTCAGTAACGTTACGGTA8510.8094662849207179No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGTGCTCCCGTA6510.619227440050984No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG6510.619227440050984No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG6380.6068619151344513No Hit
CCCGTTCGCTCCCCTGGCTTTCGTGCCTCAGCGTCAGTTTTCGTCCAGAA5510.524108017616117No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTACACTCCCGTA3820.3633561937011919No Hit
CCTGTTTGCTACCCACGCTTTCGTGCATGAACGTCAGTGTTATCCCAGGA3410.3243572305028964No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTTTGGTCCAGCA3080.2929678210993903No Hit
CCTGTTTGCTCCCCACGCTTTCGTACATGAGCGTCAGTACATTCCCAAGG2700.25682244057414083No Hit
CCCGTTCGCTCCCCTGGCTTTCGAGCCTCAGCGTCAGTTGCAGTCCAGAA2480.2358961676384701No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGTGTCAGTTGCAGTCCAGAA2450.23304258496542407No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAAGCCAGAG2390.22733541961933207No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGACCAGAG2180.20736034090800998No Hit
CCTGTTCGCTCCCCCGGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG2090.19879959288887197No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTATTATCCCAGGG2050.1949948159914773No Hit
ACTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1660.15789824124187918No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAAGCCAGAG1500.14267913365230048No Hit
CCTGTTTGATACCCGCACCTTCGTGCTTAAGCGTCAGTTGCGCTCCCGTC1450.13792316253055711No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAGCGTCAGTGTTATCCCAGGA1400.13316719140881375No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAGGG1310.12460644338967573No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTCAGCGTCAGTATCTGTCCAGTA1290.1227040549409784No Hit
CCTGTTTGCTACCCACGCTTTCGCGCATGAACGTCAGTGTTATCCCAGGA1270.12080166649228105No Hit
CCTGTTCGCTCCCCATGCTTTCGTTTCTCAGCGTCAGTTACAGCCCAGAG1260.11985047226793238No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTGCGTTCCAGCA1210.11509450114618905No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCCTCAGCGTCAGTTGTATCTTAGTG1150.10938733580009702No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG1080.10272897622965634No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTGAACGTCAGTGTTATCCCAGGA1060.10082658778095899No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGCCG700.0493.4964195
CAAACGG700.0493.4964195
ATATCCG1150.0493.4964195
ATAACCG159.210962E-7493.49637195
ATTGCCG300.0493.49637195
TAATCCG2450.0493.49637195
AAGGCCG252.3646862E-11493.49637195
AAACCGG204.6875357E-9493.49634195
GAAACCG101.8055792E-4493.49634195
CCAGCCG204.6875357E-9493.49634195
GACACCG101.8055792E-4493.49634195
GGAACCG1650.0493.49634195
ACAACCG101.8055792E-4493.49634195
CCGGCCG101.8055792E-4493.49634195
GTAACCG9250.0490.82886195
CCAACCG2100.0481.74646195
GTCACCG8200.0481.4599195
TTCGCCG1000.0468.82156195
AATCCGG251.4280886E-8394.79712195
TAGCCCG202.906425E-6370.12225195
Produced by FastQC (version 0.11.5)