FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301664

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301664
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97477
Sequences flagged as poor quality0
Sequence length197-201
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA1752017.973470664874792No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG1442414.79733680765719No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG76257.822358094730039No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG58095.959354514398268No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGTA44254.539532402515465No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG42894.400012310596345No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA37943.892200211331904No Hit
CCTGTTCGCTCCCCCGGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG31393.2202468274567333No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC24822.5462416775239287No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC21662.2220626404177395No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG18591.9071165505709038No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG17691.814787077977369No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG14761.514203350533972No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGTGCTCCCGTA12231.2546549442432573No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTTTCGTCCAGCA11831.2136196230905754No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGGCCAAAA10791.1069277880936015No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTATCGTCCAGTA9380.9622782810303969No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC8170.8381464345435333No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTACACTCCCGTA5520.5662874319070139No Hit
CCTGTTTGCTCCCCTCGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG5460.5601321337341116No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTGTCGTCCAGTA4800.492423853832186No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTGATCGTCCAGAA4580.46985442719821086No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTGCTGTCCAGTG4210.4318967551319799No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAGCGTCAGTTGTTGCCCAGAA3910.40112026426746816No Hit
CCCGTTCGCTCCCCTGGCTTTCGAGCCTCAGCGTCAGTTGCAGTCCAGAA3250.33341198436554265No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTGAGTTCCAGCA3090.31699785590446977No Hit
CCTGTTTGATCCCCACGCTTTCGAGCCTCAACGTCAGTTATTGTCCAGTA2980.30571314258748217No Hit
CCCGTTCGCTCCCCTCGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA2780.2851954820111411No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTCAGCGTCAGTATCTGTCCAGTA2400.246211926916093No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTACATTCCCAAGG2330.23903074571437363No Hit
CCTGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA2290.23492721359910543No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAAGCCAGAG1750.1795295300429845No Hit
CCTGTTTGCTCCCCTAGCTTTCGCTCCTCAGCGTCAGTTATGGCCCAGAA1680.17234834884126513No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTACCATCCAGTG1630.16721893369717986No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTTTGGTCCAGCA1620.1661930506683628No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTGCAGCCCGGAC1620.1661930506683628No Hit
CCTGTTTGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG1550.15901186946664342No Hit
CCCATTCGCTCCCCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCA1520.15593422038019225No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGTTCCCGTC1170.12002831437159535No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGTGTCAGTTATGCCTTAGTA1070.10976948408342481No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTGTCATCCCAGGA1020.10464006893933953No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCGG102.2634589E-4457.64734195
AAAGCCA206.830305E-9457.64734195
GGAACCG950.0457.64734195
CTGGCCG8650.0452.35663195
CCAGCCG2150.0447.0044195
AAGTCCG1600.0443.34586195
GTCACCG2600.0440.0455195
CCAACCG4650.0437.96362195
GAAGCCG2450.0420.28833195
ATATCCG550.0416.04303195
CAAGCCG1500.0411.8826195
TAATCCG450.0406.79764195
AAGGCCG2950.0395.59348195
CAAACCG1000.0389.00024195
CACTCCG305.1684765E-8305.09824195
GTAACCG157.6267123E-4305.09824195
CAAACGG157.6267123E-4305.09824195
TAAACCA2050.0267.8911195
TTCGCCG1000.0251.70604195
TCCACGG2850.0216.78032195
Produced by FastQC (version 0.11.5)