FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301670

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301670
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130217
Sequences flagged as poor quality0
Sequence length197-201
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA2327817.876314152530007No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG1518311.659767925846856No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG128409.860463687536958No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG96527.412242641129807No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG93737.197984902124914No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG85846.592073231605704No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC37302.86444934225178No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG34012.611794158980778No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTAACACTCCCGTA27632.121842770145219No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGGCCAAAA24661.8937619512045276No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC18831.4460477510616894No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGTA18411.4137938978781572No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGTGCTCCCGTA16261.2486848875338856No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG14031.0774322861070367No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG12640.9706873910472518No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTTTCGTCCAGCA11320.8693181381847224No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA10110.776396323060737No Hit
CCCGTTCGCTCCCCTGGCTTTCGTGCCTCAGCGTCAGTTTTCGTCCAGAA9280.712656565578995No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTCAGCGTCAGTATCTGTCCAGTA8910.684242456822074No Hit
CCTGTTTGATACCCACGCCTTCGTGCTTCAGTGTCAGTCGCAGTATGGCA8860.6804027123954629No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGTGCTCCCGCA7380.5667462773677784No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTGCTGTCCAGTG7200.5529231974319789No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTGAGTTCCAGCA6750.5183654975924802No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGCC6710.5152937020511915No Hit
CCTGTTTGCTCCCCACGCTTTCGTACATGAGCGTCAGTACATTCCCAAGG6080.4669129222758933No Hit
CCTGTTTGATCCCCACGCTTTCGAGCCTCAACGTCAGTTATTGTCCAGTA5490.4216039380418839No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGACCAGAG5340.41008470476205106No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGTGTCAGTTGCAGTCCAGAA4680.3594000783307863No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTACACTCCCGTA4380.3363616117711205No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGACCAGAG4330.33252186734450956No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTGCAGCCCGGAC4110.31562699186742127No Hit
CCTGTTCGCTCCCCATGCTTTCGTTTCTCAGCGTCAGTTACAGCCCAGAG3590.27569364983066724No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG3470.26647826320680096No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAAGCCAGAG3300.25342313215632367No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTGTCGTCCAGTA3100.23806415444987983No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTAACACTCCCGCA3070.23576030779391324No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTTTGGTCCAGCA2830.2173295345461806No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAGGG2610.20043465906909233No Hit
CCTGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA2560.19659491464248138No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG2510.19275517021587044No Hit
CCTGTTCGATACCCGCACCTTCGTGCTTAAGCGTCAGTAACGTTACGGTA2470.18968337467458166No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTACATTCCCAAGG2450.18814747690393727No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAAGCCAGAG2180.16741285700023806No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTGCAGTCCAGAA2060.15819747037637175No Hit
CCTGTTTGCTCCCCACGCTTTCGTACCTGAGCGTCAGTAAAAGTCCAGAA1870.14360644155525007No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACCTTCCCAAGG1760.13515900381670595No Hit
CCCGTTCGCTCCCCTGGCTTTCGAGCCTCAGCGTCAGTTGCAGTCCAGAA1730.1328551571607394No Hit
CCTGTTTGCTCCCCTAGCTTTCGCTCCTCAGCGTCAGTTATGGCCCAGAA1680.12901541273412842No Hit
CCTGTTTGCTACCCACGCTTTCGGGCATGAACGTCAGTGTTATCCCAGGA1480.11365643502768456No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTACAGACCAGAG1410.1082807928304292No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCGG159.3052586E-7492.41345195
GTCCCCG159.3052586E-7492.41345195
ATTGCCG159.3052586E-7492.41345195
GTAACCG300.0492.41345195
CAAACGG159.3052586E-7492.41345195
GCAACCG1450.0492.41342195
ATAACCG101.818786E-4492.41342195
CTGGCCG900.0492.41342195
CCACCCG101.818786E-4492.41342195
ATAGCCG101.818786E-4492.41342195
GATCCGG101.818786E-4492.41342195
CGAACAA750.0492.41342195
AATACCG101.818786E-4492.41342195
GGAACCG3600.0492.41342195
ATTTCCG204.7512003E-9492.41342195
CCGACCG101.818786E-4492.41342195
CGACCCG101.818786E-4492.41342195
CCGGCCG252.3646862E-11492.41342195
GTCACCG16800.0488.01685195
CAGACCG3900.0460.84845195
Produced by FastQC (version 0.11.5)