FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301688

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301688
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133261
Sequences flagged as poor quality0
Sequence length197-201
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA1566111.752125528098993No Hit
CCCGTTCGCTCCCCTGGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAA106537.9940867920847065No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG95007.128867410570234No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC90806.8136964303134455No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG79595.972490075866157No Hit
CCTGTTCGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTATCGTCCAGTG71585.37141399209071No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC53113.985412086056686No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG45743.4323620564156054No Hit
CCTGTTCGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCACTCCGGAC43263.24626109664493No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGTGCTCCCGTA40533.041399959478017No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG35502.663945190265719No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTACACTCCCGTA34592.595658144543415No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTGCAGCCCGGAC33282.4973548149871303No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG30962.3232603687500473No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGTA28342.1266537096374782No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTATCGTCCAGTA24211.8167355790516357No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC21111.5841093793382908No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA17581.319215674503418No Hit
CCCGTTCGCTCCCCTGGCTTTCGAGCCTCAGCGTCAGTTGCAGTCCAGAA16001.2006513533591974No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA14791.109852094761408No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTGCTGTCCAGTG14491.0873398818859232No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAGCGTCAGTTGTTGCCCAGAA14221.0670788902979866No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTGTCGTCCAGTA13130.9852845168503913No Hit
CCTGTTTGCTCCCCTAGCTTTCGCTCCTCAGCGTCAGTTATGGCCCAGAA12290.9222503207990335No Hit
CCTGTTCGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTGCCGTCCAGTG11760.88247874471901No Hit
CCTGTTTGCTCCCCAAACTGTCGTCCCTCATCGTCAAGTATTCTATAGTT10700.8029355925589633No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTTTCGTCCAGCA10340.7759209371083814No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA9730.7301461042615619No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTGATCGTCCAGAA9380.7038818559068295No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTGAGTTCCAGCA9240.6933761565649366No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGCCAGTTACCGTCCAGTG6860.5147792677527558No Hit
CCTGTTTGATCCCCACGCTTTCGAGCCTCAACGTCAGTTATTGTCCAGTA6410.4810109484395284No Hit
CCTGTTTGCTACCCGAACTGTCGTCCCTCATCGTCAAGTATTCTGTAGTT6290.4720060632893344No Hit
CCTATTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCTTTGGCTAGCA5900.44274018655120406No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAA5340.4007173891836321No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTTAGTTATCGTCCAGTA5160.38721006145834114No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTAAGTTCTAGCA4580.34368644989907027No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTAACGCTCCCGCA3940.2956603957647024No Hit
CCCGTTCGCTCCCCTGGCTTTCGAGCCTCAACGTCAGTTGCAGTCCAGAA3490.2618920764514749No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTATCGTCCAGTT3470.26039126225977594No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGAA2950.22137009327560203No Hit
CCTGTTTGATACCCGCACCTTCGAGCCTCAGCGTCAGTTACGCTCCGGAT2640.1981074733042676No Hit
CCTGTTCGATACCCGCACCTTCGAGCCTCAGCGTCAGTTACGCTCCGGAT1990.14933101207405017No Hit
CCTGTTCGCTCCCCCGGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG1850.1388253127321572No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGTGTCAGTTATGCCTTAGTA1780.1335724630612107No Hit
CCTGTTTGCTCCCCTCGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG1700.12756920629441473No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTACCATCCAGTG1570.11781391404837124No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTTTGGTCCAGCA1480.11106025018572577No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTACAGTCCAGAA1410.10580740051477927No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTGCTGTCCAGTA1380.10355617922723079No Hit
CCTGTTCGCTCCCCTCGCTTTCGCACTTCAGCGTCAGTTATCGTCCAGTG1370.10280577213138127No Hit
CCTGTTCGATACCCGCACCTTCGCGCTTAAGCGTCAGTTGCGCTCCCGTC1370.10280577213138127No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACCG5550.0331.77863195
TATGCGG105.9431954E-4331.77863195
TAGCCCG105.9431954E-4331.77863195
TCTGCGG252.6011548E-10331.77863195
AAAACCG301.8189894E-12331.77863195
ATCGCCG154.5133456E-6331.77863195
GTAACCG203.4211553E-8331.77863195
CTGGCCG18100.0330.86212195
TCGTCCG4500.0324.40576195
TCTCCGG13700.0323.30255195
CCGACCG850.0312.26224195
TCCACGG4050.0303.1064195
CCGGCCG1100.0301.61694195
ATATCCG1200.0290.3063195
CCAGCCG1850.0277.9767195
TATCCAG307.767085E-10276.4822195
TAATCCG2000.0273.71738195
GAAGCCG6200.0272.91467195
TTCGCCG1850.0224.17477195
GCAGCCG150.0020013095221.18576195
Produced by FastQC (version 0.11.5)