FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301692

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301692
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179766
Sequences flagged as poor quality0
Sequence length197-201
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG3109517.297486732752578No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG2856315.888989019058108No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG2153811.981131025889212No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1982911.030450697017233No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA127367.084765751031898No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG107205.963307855768054No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG104805.829800963474739No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGGCCAAAA95795.3285938386569205No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG32971.8340509328794097No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC27531.5314353103478968No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG24441.3595451865202541No Hit
CCTGTTTGCTACCCACGCTTTCGGGCATGAACGTCAGTGTTATCCCAGGA24411.3578763503665876No Hit
CCTGTTTGCTCCCCCGGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG10940.6085689173703592No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGTGCTCCCGTA9550.5312461755838145No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC7420.41275880867349773No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTCAGCGTCAGTATCTGTCCAGTA6070.3376611817585083No Hit
CCTGTTCGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG5630.3131849181714006No Hit
CCTGTTCGCTACCCACGCTTTCGAGCCTCAGCGTCAGTGACAGACCAGAG4810.2675700633045181No Hit
CCTGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA4050.22529288074496848No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTAACTGCCCAGTA3890.21639242125874747No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTTACTGCCCAGAG3800.2113859127977482No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCATCAGCGTCAATTAATTGTTAGTA3340.1857970917748629No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGACCAGAG3150.17522779613497547No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCATCAGCGTCAATAAATTGTTAGTA3120.17355895998130902No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACCTTCCCAAGG3110.17300268126342022No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG3010.1674398940845321No Hit
CCCGTTCGCTCCCCTGGCTTTCGAGCCTCAGCGTCAGTTGCAGTCCAGAA2900.1613208281877552No Hit
CCCATTTGCTCCCCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCA2730.1518640899836454No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGTACTCCCGTA2600.14463246665109086No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTTACAGCCCAGAG2490.13851340075431395No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTGACAGACCAGAG2420.13461944972909226No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGGCCAAAA2380.13239433485753702No Hit
CCTGTTTGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG2210.12293759665342724No Hit
CCTGTTCGCTCCCCACGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG2180.1212687604997608No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG2110.11737480947453913No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTTACAGCCCAGAG1900.1056929563988741No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGCCG1150.0465.64227195
GGCACCG102.1524692E-4465.64224195
GAAACCG400.0465.64224195
CCAGCCG1300.0465.64224195
GCAACCG102.1524692E-4465.64224195
CTGGCCG102.1524692E-4465.64224195
AAGACCG102.1524692E-4465.64224195
TAATCCG151.1655065E-6465.64224195
CCGGCCG1850.0465.64224195
CCGGTCG102.1524692E-4465.64224195
TAAACCA11200.0465.6422195
AGGGCCG350.0465.6422195
GTAACCG2850.0465.6422195
GTCACCG30550.0464.88013195
GGAACCG8700.0462.9661195
CCAACCG3900.0435.79337195
TTGGCCG251.918852E-8372.5138195
ATAACCG157.25292E-4310.42816195
AAAGCCA157.25292E-4310.42816195
TCAACCA258.962346E-6279.38535195
Produced by FastQC (version 0.11.5)