FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301702

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301702
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160294
Sequences flagged as poor quality0
Sequence length197-201
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG2584516.123498072292165No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA2250514.039826818221519No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG129428.073914182689307No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG84945.299013063495826No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC82265.131820280235068No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG74164.626498808439492No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG69514.3364068524086985No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGTA65964.114938799955083No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTGTTATCCCAGGA48793.043782050482239No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG47282.9495801464808418No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC47122.9395984877787065No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC43012.6831946298676184No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG19751.2321109960447678No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGGCCAAAA14700.9170648932586373No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGTGCTCCCGTA14530.9064593808876189No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTACACTCCCGTA11190.6980922554805545No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG10760.6712665477185672No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA10570.659413328009782No Hit
CCCGTTCGCTCCCCTGGCTTTCGAGCCTCAGCGTCAGTTGCAGTCCAGAA10330.6444408399565797No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGACCAGAG10180.6350830349233284No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTGATCGTCCAGAA9910.6182389858634759No Hit
CCCGTTCGCTCCCCTGGCTTTCGTGCCTCAGCGTCAGTTTTCGTCCAGAA8530.532147179557563No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTGCAGCCCGGAC7640.47662420302693803No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGCGTCAGTTGTACCTTAGTA6870.4285874705229142No Hit
CCTGTTTGCTCCCCTAGCTTTCGCTCCTCAGCGTCAGTTATGGCCCAGAA6600.4117434214630616No Hit
CCTGTTCGCTCCCCCGGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG6370.3973947870787429No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTGTCGTCCAGTA6370.3973947870787429No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG6050.37743146967447316No Hit
CCTGTTTGATCCCCACGCTTTCGAGCCTCAACGTCAGTTATTGTCCAGTA5000.3119268344417133No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTGCTGTCCAGTG4950.3088075660972962No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAAGCCAGAG4640.28946810236191No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG4270.26638551661322324No Hit
CCTGTTTGCTACCCACGCTTTCGGGCATGAACGTCAGTGTTATCCCAGGA4160.2595231262555055No Hit
CCTGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA3970.2476699065467204No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTGAGTTCCAGCA3750.23394512583128502No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTATCGTCCAGTA3470.21647722310254905No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTTTCGTCCAGCA3380.21086254008259825No Hit
CCTGTTCGCTCCCCATGCTTTCGTTTCTCAGCGTCAGTTACAGCCCAGAG3080.19214693001609542No Hit
CCTGTTCGCTCCCCACGCTTTCGTTCCTCAGCGTCAGTAACGGCCCAGAG2970.1852845396583777No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAAGCCAGAG2960.1846606859894943No Hit
CCTGTTCGATACCCGCACCTTCGTGCTTAAGCGTCAGTTGCGCTCCCGTC2920.1821652713139606No Hit
CCTGTTCGCTCCCCACGCTTTCGTTCCTCAGCGTCAGTAACGGCCCAGTA2630.16407351491634123No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGACCAGAG2530.15783497822750694No Hit
CCTATTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCTTTGGCTAGCA2160.13475239247882018No Hit
CCTGTTCGATACCCGCACCTTCGTGCTTAAGCGTCAGTAACGTTACGGTA2150.13412853880993672No Hit
CCTGTTCGATACCCGCACCTTCGCGCTTAAGCGTCAGTTGCGCTCCCGTC2150.13412853880993672No Hit
CCTGTTTGCTCCCCACGCTTTCGTACATGAGCGTCAGTACATTCCCAAGG2140.13350468514105332No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTATCCCAGGA2090.13038541679663618No Hit
CCTGTTTGCTACCCACGCTTTCGTGCATGAACGTCAGTGTTATCCCAGGA2080.12976156312775275No Hit
CCTGTTCGATACCCGCACCTTCGTGCTTCAGCGTCAGTTGCGCTCCCGTC1930.12040375809450135No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGTGCTCCCGCA1910.11915605075673451No Hit
CCCGTTCGCTCCCCTGGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAA1830.11416522140566708No Hit
CCTGTTCGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCACTCCGGAC1800.11229366039901681No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG1730.10792668471683282No Hit
CCTGTTTGATACCCGCACCTTCGTGCTTAAGCGTCAGTTGCGCTCCCGTC1630.10168814802799855No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGTGTCAGTTGCAGTCCAGAA1620.10106429435911514No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGCGG254.0017767E-11455.17825195
AATCCGG400.0455.17825195
CCACCCG254.0017767E-11455.17825195
ATTGCCG102.3037431E-4455.17825195
GATCCGG102.3037431E-4455.17825195
ATATCCG650.0455.17825195
ATAACCG300.0455.17822195
TGAACCG151.2757919E-6455.17822195
GTAACCG7450.0455.17822195
GGAACCG1100.0455.17822195
GTCACCG4650.0450.28387195
CCAGCCG6650.0438.06628195
CTGGCCG2600.0437.6714195
CCAACCG6850.0421.95352195
GAAGCCG2500.0418.76398195
CGAACCG500.0409.66043195
TAATCCG800.0398.28098195
GCAACCG350.0390.1528195
CAAACGG350.0390.1528195
CCGGCCG350.0390.1528195
Produced by FastQC (version 0.11.5)