FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301725

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301725
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135779
Sequences flagged as poor quality0
Sequence length197-201
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA2190816.13504297424491No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGCGCA1998414.718034453045023No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA1810413.33343153212205No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA1571411.573218244352956No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA108547.993872395584001No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA100377.392159317714817No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA57434.22966732705352No Hit
TACGTATGTCACAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGTCTA41033.0218222258228447No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA39242.889990351969008No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA35912.6447388771459504No Hit
TACGTATGTCGCAAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA30422.2404053645998276No Hit
TACGTATGTCGCGAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA28422.0931071815229156No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA15651.1526082825768345No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA11080.8160319342460911No Hit
TACGGAGGGTGCAAGCGTTACTCGGAATCACTGGGCGTAAAGGACGCGTA8860.652530951030719No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTCCGTA6810.5015503133768845No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA4570.33657634833074335No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA3350.24672445665382714No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGTGTA3170.23346762017690512No Hit
TACGGGGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA2980.21947429278459848No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGGGCGCA2230.1642374741307566No Hit
TACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA2150.15834554680768012No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCGCGCA2020.14877116490768086No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA1660.12225749195383674No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA1630.12004801920768307No Hit
TACGTATGTCACAAGCGTTATCCGGATTTATTGGGCGTAAAGTGCGTCTA1570.11562907371537573No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTCATTGGGCGTAAAGCGCTCGTA1510.11121012822306836No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCTTGTA1460.10752767364614559No Hit
TACGGAAGGTTCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA1460.10752767364614559No Hit
TACATATGGGGCGAGCGTTATCCGGATTTATTGGGTGTAAAGGGTGCGTA1370.10089925540768455No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGCAG108.4305747E-4295.2503195
AAGATAG6100.0295.25027195
AAGGCAT255.2386895E-10295.25027195
AAGGCAG53950.0294.42938195
AAGCGGG5200.0283.8945195
AAGGCGG12300.0266.44537195
ACGGCAG251.864646E-7236.20021195
GTGCGAG39900.0194.658589
GTATGGT204.899466E-7194.658574
TCCCAAG100.0029357297194.658579
GTCACAA4800.0194.658578
GTCGCGA3150.0194.658578
GGCGCGA10300.0194.658578
TGTCCCG100.0029357297194.658577
TGTCCCA100.0029357297194.658577
GGTCCGG1150.0194.658578
GTCCGGG1150.0194.658579
TATGTCC204.899466E-7194.658575
TACGGAG20850.0194.658571
TACGGAA13500.0194.658571
Produced by FastQC (version 0.11.5)