FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151276
Sequences flagged as poor quality0
Sequence length195-201
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA7251647.93622253364711No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA2406215.906026071551338No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA2296215.178878341574343No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA108937.200745656944922No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA44532.943626219625056No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA32862.1721885824585523No Hit
TACGGAGGGTGCAAGCGTTACTCGGAATCACTGGGCGTAAAGGACGCGTA25471.6836775165921893No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA25261.6697956053835374No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA4230.27962135434569924No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA3660.2419418810650731No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA3630.2399587508924086No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA2620.1731933684127026No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCTTGTA1870.12361511409608927No Hit
TACGTAGGTCCCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA1690.11171633306010208No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGCA103.912969E-5821.76227195
AAGGCAG8550.0812.15094195
AAGGCGG7050.0780.9655195
AAGCGGG1750.0774.8044195
AAGGAGG151.3194275E-4547.8415195
GAAGGCA20200.0475.9712195
AAGGGGG1150.0250.10155195
GTAGGTC99950.0194.234734
TAGGGGG550.0194.234735
GTCGCGA450.0194.234718
GTCGCAA100.0029552728194.234718
GTTCCCG153.829718E-5194.234717
GGTCCCG99150.0194.234717
TACGGAA4450.0194.234711
TCGCGAG450.0194.234719
AGGGCGC12600.0194.234716
AGGGTGC6700.0194.234716
AGGGCCC100.0029552728194.234716
ACGTAGT204.954836E-7194.234712
CGTAGTT204.954836E-7194.234713
Produced by FastQC (version 0.11.5)