FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301734

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301734
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151276
Sequences flagged as poor quality0
Sequence length197-201
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG6399942.3061159734525No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA1733811.461170311219227No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG146599.6902350670298No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG106857.06324863164018No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG58803.886935138422486No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGGCCAAAA54103.5762447447050425No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC41652.753245723049261No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAA41612.7506015494857086No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG30622.0241148628996006No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG23141.5296544065152435No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTGAGTTCCAGCA21471.419260160236918No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG11980.7919299822840372No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG4860.3212670879716545No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTGATCGTCCAGAA4330.28623178825458107No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTTTCGTCCAGCA3490.23070414341997408No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG2860.1890584097940189No Hit
CCTGTTCGCTCCCCACGCTTTCGATCCTCAGCGTCAGTTACAGACCAGAG2590.1712102382400381No Hit
CCTGTTTGATCCCCACGCTTTCGAGCCTCAACGTCAGTTATTGTCCAGTA2350.1553451968587218No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACACGCCAGAG2270.15005684973161637No Hit
CCTGTTCGATCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1890.12493720087786563No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTGCGTTCCAGCA1640.10841111610566119No Hit
CCTGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA1530.10113963880589122No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCGG50.00300952681647.2051195
TCAACCA50.00300952681647.2051195
AGAACCG50.00300952681647.2051195
TAATCGG50.00300952681647.2051195
TCACCCA50.00300952681647.2051195
GTAACCG50.00300952681647.2051195
GGAACCG1600.01647.2051195
CATACCG50.00300952681647.2051195
TAATCCG2600.01615.5281195
TCTCCAG4400.01609.7686195
TAAACCA5150.01599.2283195
GTCACCG2300.01575.5875195
CATTCCG203.870375E-5823.60254195
ATAACCG455.9561717E-7549.06836195
CCAACCG352.071472E-4470.63004195
AAACCGA351.7340622E-5235.31502195
TAGCTCC256.521077E-9193.85296
ATACCCA256.521077E-9193.85299
CGATCCC500.0193.85297
ATCCCCA700.0193.852899
Produced by FastQC (version 0.11.5)