FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301751

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301751
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69187
Sequences flagged as poor quality0
Sequence length198-201
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA1509221.81334643791464No Hit
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGTA906213.097836298726639No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGTA816411.799904606356685No Hit
TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA59208.556520733663838No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA44076.369693728590631No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA43326.261291861187796No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA37855.470680908263113No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA35775.170046395999249No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA32844.746556434012169No Hit
TACGTAGGGGGCGAGCGTTATCCGGATTCATTGGGCGTAAAGCGCGCGTA24393.5252287279402204No Hit
TACGTAGGGTGCAAGCGTTGTCCGGAATTACTGGGCGTAAAGAGCTCGTA20803.006345122638646No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA14942.159365198664489No Hit
TACGAAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA8961.2950409759058783No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGTA2050.2962984375677512No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGAGCGCA1220.1763337043086129No Hit
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGCGCGTA1090.15754404729212135No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGCGTA840.12141009149117608No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA840.12141009149117608No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTCATTGGGCGTAAAGCGCGCGTA740.10695650917079798No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTCATTGGGCGTAAAGCGCGCGTA740.10695650917079798No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGCAG28750.0208.29965195
AAGGCGA8950.0207.49683195
AAGGCGG26250.0204.29057195
GGTCCCG4050.0194.932777
GTCCCGA4050.0194.932778
TCCCGAG4050.0194.932779
ACAGCGG100.002920302194.932752
GAGGTCC100.002920302194.932755
AGGGGGC2300.0194.932756
CGGAGGT256.2718755E-9194.932753
GTGCAAG17550.0194.932759
ACAGAGG15400.0194.932752
TACAGCG100.002920302194.932751
TACAGAG15400.0194.932751
ACGTCGG100.002920302194.932752
TCGGTGG100.002920302194.932755
TACGGAG10800.0194.932751
ATGGTGC153.766505E-5194.932756
AGGTGGC28900.0194.932756
CGGGTGC153.766505E-5194.932756
Produced by FastQC (version 0.11.5)