FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301754

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301754
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137543
Sequences flagged as poor quality0
Sequence length198-201
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG2796320.330369411747597No Hit
CCTGTTTGCTCCCCATGCTTTCGTACCTCAGCGTCAGTATTAGGCCAGAT1731512.58879041463397No Hit
CCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAA1621511.789040518237933No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGTGTCAGTATGATGCCAGGG126349.185491082788655No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG85466.213329649636841No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG80145.826541517925303No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG78765.7262092581956185No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCTTGGCA73305.329242491439041No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG58044.2197712715296305No Hit
CCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAC54403.9551267603585787No Hit
CCTGTTTGCTACCCATGCTTTCGCTCCTCAGCGTCAGTAACTGCCCAGTA40892.972888478512174No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA28592.078622685269334No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT16521.2010789353147744No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCTTGGGA6300.4580385770268207No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG3410.2479224678827712No Hit
CCTGTTCGCTCCCCCCGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG2410.17521793184676793No Hit
CCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAG2280.1657663421620875No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAA2130.154860661756687No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGCGTCAGTATGATGCCAGGG1960.14250089063056642No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAA1920.1395927091891263No Hit
CCTGTTTGATCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1920.1395927091891263No Hit
CCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1650.11996248445940542No Hit
CCTGTTTGCTCCCCATGCTTTCGCACCTCAGCGTCAGTATTAGGCCAGAT1530.11123794013508503No Hit
CCTGTTTGATCCCCACGCTTTCGCACCTCAGCGTCAGTTACAGACCAGAA1400.10178635045040461No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAACCG26950.0246.06155195
CGGGCCG4450.0246.06155195
CAAACCA4600.0246.06155195
ACAACCG301.6370905E-11246.06155195
GAAACCA100.0014554102246.06154195
CAACCCA100.0014554102246.06154195
CGAACCG6600.0246.06154195
AAAACCG19900.0245.44331195
ATAACCA12250.0245.05722195
TGAACCA2050.0240.06004195
AAAGCCG18800.0239.51736195
AAGGCCG2050.0234.05855195
GCAACCG354.7293724E-11210.9099195
ACTGTTT308.185452E-11194.791291
GTTTGAT31400.0194.791284
CCTGTTT107900.0194.791281
GATACCC3100.0194.791288
TGATCCC28350.0194.791287
TGTTCGC28750.0194.791283
TGTTTAC100.0029297897194.791283
Produced by FastQC (version 0.11.5)