FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301764

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301764
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80998
Sequences flagged as poor quality0
Sequence length197-201
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA1246515.389268870836318No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC62037.658213783056373No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG52836.522383268722684No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG52616.495222104249487No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGTGCTCCCGTA44485.491493617126348No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC40545.005061853379096No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG30603.777871058544655No Hit
CCCGTTCGCTCCCCTGGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAA26353.253166744857898No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG20022.471665967060915No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG19952.4630237783648976No Hit
CCCGTTCGCTCCCCTGGCTTTCGAGCCTCAGCGTCAGTTGCAGTCCAGAA18142.2395614706535962No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTACACTCCCGTA17232.127213017605373No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTGATCGTCCAGAA16812.0753598854292696No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC16612.0506679177263636No Hit
CCTGTTCGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCACTCCGGAC11961.4765796686337933No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTGTTATCCCAGGA11121.372873404281587No Hit
CCTGTTCGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTATCGTCCAGTG10221.2617595496185092No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTATCGTCCAGTA9951.2284253932195857No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG9921.2247215980641497No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTGAGTTCCAGCA9041.1160769401713624No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG8701.0741005950764217No Hit
CCTGTTTGCTACCCACGCTTTCGGGCATGAACGTCAGTGTTATCCCAGGA8361.0321242499814811No Hit
CCCGTTCGCTCCCCTGGCTTTCGCACCTCAGCGTCAGTTTTCGTCCAGAA6410.7913775648781451No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTAACGCTCCCGCA5430.6703869231339046No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTGTCGTCCAGTA5400.6666831279784685No Hit
CCTGTTCGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTGCCGTCCAGTG5000.6172991925726562No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGTA5000.6172991925726562No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAGCGTCAGTTGTTGCCCAGAA4710.5814958394034421No Hit
CCCGTTCGCTCCCCTGGCTTTCGTGCCTCAGCGTCAGTTTTCGTCCAGAA4670.5765574458628608No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG3930.4851971653621078No Hit
CCTGTTTGCTCCCCTAGCTTTCGCTCCTCAGCGTCAGTTATGGCCCAGAA3800.46914738635521863No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTGCAGCCCGGAC3550.43828242672658585No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTGCTGTCCAGTG3370.4160596557939702No Hit
CCTGTTCGATACCCGCACCTTCGTGCTTCAGCGTCAGTTGCGCTCCCGTC3090.3814909010099015No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTTTCGTCCAGCA2740.33827995752981554No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGGCCAAAA2550.3148225882120546No Hit
CCTGTTTGATCCCCACGCTTTCGAGCCTCAACGTCAGTTATTGTCCAGTA2500.3086495962863281No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTATCGTCCAGTG2400.2963036124348749No Hit
CCTGTTCGCTCCCCACGCTTTCGTTCCTCAGCGTCAGTAACGGCCCAGAG2340.28889602212400306No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGTGCTCCCGTA2320.28642682535371244No Hit
CCTGTTTGCTCCCCTAGCTTTCGTACTTCAGCGTCAGTTACCGTCCAGTG2200.2716116447319687No Hit
CCTGTTTGCTCCCCTCGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG2100.25926566088051556No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC1830.22593150448159213No Hit
CCTGTTCGATACCCGCACCTTCGTGCTTAAGCGTCAGTTGCGCTCCCGTC1780.21975851255586556No Hit
CCTGTTCGATACCCGCACCTTCGCGCTTCAGCGTCAGTTGCGCTCCCGTC1780.21975851255586556No Hit
CCTGTTTGATACCCGCACCTTCGTGCTTCAGCGTCAGTTGTGCTCCCGTA1620.20000493839354058No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTACTGTCCAGTG1600.19753574162324994No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTATCGTCCAGTT1290.15926319168374528No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA1160.14321341267685622No Hit
CCCGTTCGCTCCCCTGGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAA1150.14197881429171091No Hit
CCTGTTTGATACCCGCACCTTCGCGCTTCAGCGTCAGTTGTGCTCCCGTA1110.13704042075112965No Hit
CCTATTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCTTTGGCTAGCA1060.1308674288254031No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGACCAGAG1050.12963283044025778No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTGCCGTCCAGTG1010.12469443689967652No Hit
CCTGTTCGATACCCGCACCTTCGCGCTTAAGCGTCAGTTGCGCTCCCGTC980.1209906417442406No Hit
CCCGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA960.11852144497394998No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTCAGCGTCAGTATCTGTCCAGTA900.1111138546630781No Hit
CCTGTTCGATACCCGCACCTTCGTGCTTAAGCGTCAGTTACACTCCCGTA820.10123706758191561No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAA820.10123706758191561No Hit
CCGGTTCGCTCCCCACGCTTTCGTGCCTTAGCGTCAGAAATGGCCCAGTA820.10123706758191561No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGACCAGAG810.10000246919677029No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTAACTGCCCAGTA810.10000246919677029No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACCG5050.0320.84012195
GTCACCG750.0320.84012195
TATGCGG106.563581E-4320.84012195
TCTGCGG155.1481056E-6320.84012195
GCAGCCG106.563581E-4320.84012195
CGAACCG1300.0320.84012195
TCGTCCG1200.0320.84012195
AAAACCG155.1481056E-6320.84012195
GGAACCG400.0320.84012195
CCGGCCG800.0320.84012195
CTGGCCG7100.0318.5807195
GTAACCG2300.0306.89053195
TAATCCG1100.0306.25647195
TCTCCGG9600.0299.11658195
GAAGCCG1400.0286.46442195
TCCACGG5100.0286.23972195
TTCGCCG900.0267.36676195
AAGTCCG309.440555E-10267.36676195
AATCCGG201.6230213E-5240.63008195
CAAGCCG1600.0240.63008195
Produced by FastQC (version 0.11.5)