FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301780

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301780
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131652
Sequences flagged as poor quality0
Sequence length197-201
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG5646542.88958770090846No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1929214.653784219001611No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG1673212.709263816728953No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGGCCAAAA39643.010968310394069No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG37512.8491781362987267No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA33512.5453468234436243No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTGTTATCCCAGGA23441.7804514933309028No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG22971.7447513140704278No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTTACTGCCCAGAG12980.9859326102148087No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGTA11990.9107343602831709No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG10380.7884422568589918No Hit
CCTGTTCGCTACCCACGCTTTCGAGCCTCAGCGTCAGTGACAGACCAGAG8590.6524777443563333No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG8550.6494394312277824No Hit
CCCATTTGCTCCCCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCA8540.6486798529456446No Hit
CCCATTCGCTCCCCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCA7840.5955093731960016No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTAACTGCCCAGTA7010.5324643757785678No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG6140.4663810652325829No Hit
CCGGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGAAGTGGACCAGGT3380.2567374593625619No Hit
CCTGTTCGCTCCCCACGCTTTCGATCCTCAGCGTCAGTTACAGACCAGAG3310.2514204113875976No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC3270.24838209825904659No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC3200.24306505028408226No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACAGGCCCAGGG2990.22711390635918935No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG2960.22483517151277613No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACAGCCCAGAG2680.2035669796129189No Hit
CCTGTTCGCTCCCCACGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG2330.17698173973809742No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGCAGGCCCAGGG2250.17090511348099535No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG2170.1648284872238933No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTGACAGACCAGAG1690.12836872968128096No Hit
CCTGTTTGCTACCCACGCTTTCGGGCATGAACGTCAGTGCTATCCCAGGG1480.11241758575638806No Hit
CCTGTTCGATCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1470.11165800747425031No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGCGTCAGTTGTACCTTAGTA1460.11089842919211254No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTACACGGCCCAGA1350.10254306808859721No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGTTG50.00462126831329.3516195
GAAGCCG151.7400453E-81329.3516195
AGGGCCG50.00462126831329.3516195
ACATCCA50.00462126831329.3516195
CTGGCGG50.00462126831329.3516195
CTGGCCG151.7400453E-81329.3516195
TAACCCA50.00462126831329.3516195
CCATCCG50.00462126831329.3516195
TTAACCA50.00462126831329.3516195
GGAACCG500.01329.3516195
ATATCCG400.01329.3516195
CGTCCCG50.00462126831329.3516195
ACAACCG250.01329.3516195
TAAACCA4250.01313.7123195
GTAACCG2900.01306.4318195
CCAACCG4150.01297.3191195
CCAGCCG1000.01262.8839195
AAAGCCA1750.01253.3887195
GTCACCG700.01234.3978195
CGAACCG350.0949.5368195
Produced by FastQC (version 0.11.5)