FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301787

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301787
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142833
Sequences flagged as poor quality0
Sequence length198-201
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA2781019.470290479090966No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA2323816.26934952006889No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA2295416.070515917190008No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA1565710.961752536178613No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA131989.240161587308256No Hit
TACGTATGTCGCAAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA84395.908298502446914No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCATAAAGCGCGCGCA38612.7031568335048624No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA35432.480519207746109No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA34982.449013883346286No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA26361.845511891509665No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA19621.3736321438323076No Hit
TACGGAGGGTGCAAGCGTTACTCGGAATCACTGGGCGTAAAGGACGCGTA17651.23570883479308No Hit
TACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA10060.7043190299160558No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCGCGCA8440.590899862076691No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTCATTGGGCGTAAAGCGCTCGTA7660.5362906331169968No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGAGTGCGTA5560.38926578591782013No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA5150.36056093479798085No Hit
TACGGAAGGTCCTGGTGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA4750.3325562019981377No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA4690.3283554920781612No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGAGCGCA4140.2898489844783768No Hit
TACGTATGTCGCGAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA3840.2688454348784945No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGCGCA2620.18343099983897276No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA2600.18203076319898065No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATCATTGGGCGTAAAGGGAGCGCA2340.16382768687908256No Hit
TACGTAGGTCCCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA1610.11271904951936879No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGGGTGCGTA1470.10291739303942367No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCGGG1700.0390.0885195
AGGGCGG103.6583695E-4390.08847195
AAGGTAG1600.0390.08847195
AAGATAG9100.0387.94513195
AAGGCAG38250.0386.51904195
AGGCAGG254.6407877E-8312.07077195
AAGGCGG11600.0290.88498195
ACGGCAG303.7583923E-5195.04425195
GTATGTC8850.0194.497914
GAGGTCC100.0029431516194.497915
GTCACAA153.8086506E-5194.497918
GAGGATG100.0029431516194.497915
ACGTCGG100.0029431516194.497912
AGGATGC100.0029431516194.497916
TGTCCCG100.0029431516194.497917
GTCGCAA8300.0194.497918
GCAGGTC153.8086506E-5194.497915
GAAGTTC100.0029431516194.497915
GGTCCCG42250.0194.497917
TATGTCG8650.0194.497915
Produced by FastQC (version 0.11.5)