FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301792

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301792
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118209
Sequences flagged as poor quality0
Sequence length197-201
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA2908024.600495732135457No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1228210.390071821942492No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG113529.603329695708448No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTGCTGTCCAGTG68045.755906910641322No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC54184.583407354769942No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG52134.409985703288244No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTGATCGTCCAGAA50164.24333172601071No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGTA44993.805970780566623No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC38363.245099780896547No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC35933.0395316769450718No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG33642.845807002850883No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA21501.8188124423690242No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG21031.779052356419562No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTGTTATCCCAGGA19251.628471605376917No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTAATCGTCCAGAA17101.4465903611400148No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG14461.2232571124026088No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG12431.0515273794719522No Hit
CCTGTTCGCTCCCCCGGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG10150.8586486646532837No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG8600.7275249769476098No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTGTCGTCCAGTA8410.7114517507127207No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTTTCGTCCAGCA8010.6776133796919016No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTCAGCGTCAGTATCTGTCCAGTA6160.5211109137206135No Hit
CCTGTTTGCTCCCCTAGCTTTCGCTCCTCAGCGTCAGTTATGGCCCAGAA5990.5067296060367654No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTGAGTTCCAGCA5780.4889644612508354No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGGCCAAAA4320.36545440702484583No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTACACTCCCGTA4170.3527650178920387No Hit
CCTGTTTGATCCCCACGCTTTCGAGCCTCAACGTCAGTTATTGTCCAGTA3860.3265402803509039No Hit
CCTGTTTGCTACCCACGCTTTCGGGCATGAACGTCAGTGTTATCCCAGGA3830.3240024025243425No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGTGCTCCCGTA3630.307083217013933No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAA2610.22079537091084434No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTATCCCAGGA2430.2055681039514758No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTTTGGTCCAGCA2430.2055681039514758No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGACGGAGCCTGGTA2170.1835731627879434No Hit
CCTGTTTGCTCCCCACGCTTTCGTACCTGAGCGTCAGTAAAAGTCCAGAA1880.15904034379784956No Hit
CCTGTTTGCTACCCACGCTTTCGGGCATGAACGTCAGTGCCATCCCAGGG1850.15650246597128814No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGTGCTCCCGCA1740.1471969139405629No Hit
CCTGTTTGATACCCGCACCTTCGAGCCTCAGCGTCAGTTACGCTCCGGAT1520.12858580987911242No Hit
CCTGTTTGCTCCCCTCGCTTTCGCACTTCAGCGTCAGTTGCTGTCCAGTG1450.12266409495046908No Hit
CCTGTTCGATACCCGCACCTTCGAGCCTCAGCGTCAGTTACGCTCCGGAT1370.11589642074630528No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG1330.11251258364422337No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGAA1270.10743682799110049No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGCCG102.1176387E-4468.01935195
GTCACCG400.0468.01935195
CCAGCCG3750.0468.01935195
CATCCAG102.1176387E-4468.01935195
ATAACCG151.1396296E-6468.01935195
CTGGCCG1850.0468.01935195
TCTGCGG102.1176387E-4468.01935195
ATTGCCG102.1176387E-4468.01935195
CACTCCG102.1176387E-4468.01935195
GGGGCGG151.1396296E-6468.01935195
GTAACCG2800.0468.01935195
GGAACCG1200.0468.01935195
AAGTCCG300.0468.01935195
GCAACCG500.0468.01932195
CCAACCG6550.0464.44666195
GAAGCCG4000.0462.1691195
ATATCCG6600.0460.9281195
TACGCCA309.822543E-11390.0161195
TCTCCGG5700.0381.80527195
TAATCCG1000.0374.41547195
Produced by FastQC (version 0.11.5)