FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301798

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301798
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70296
Sequences flagged as poor quality0
Sequence length197-201
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1146416.308182542392167No Hit
CCTGTTTGCTCCCCATGCTTTCGTACCTCAGCGTCAGTATTAGGCCAGAT913212.990781836804368No Hit
CCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAA748510.647832024581769No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGTGTCAGTATGATGCCAGGG55947.957778536474337No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG49026.97336975076818No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG39585.630476840787527No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCTTGGCA36955.256344599977239No Hit
CCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAC34424.896437919654034No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG30614.354444065096165No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG26093.711448731080005No Hit
CCTGTTTGCTACCCATGCTTTCGCTCCTCAGCGTCAGTAACTGCCCAGTA21993.1282007511095937No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA13111.8649709798566065No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT12011.7084898144986913No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCTTGGGA5730.815124615909867No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGACCAGAG4310.6131216569932856No Hit
CCTGTTTGATCCCCACGCTTTCGTACATCAGCGTCAGTTACAGACCAGAA2590.36844201661545467No Hit
CCTGTTTGCTCCCCATGCTTTCGCACCTCAGCGTCAGTATTAGGCCAGAT2350.334300671446455No Hit
CCTGTTTGCTACCCACGCTTTCGTACCTCAGTGTCAGTATGATGCCAGGG2240.31865255491066347No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG2100.298736770228747No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGACCAGAG2030.2887788778877888No Hit
CCTGTTTGCTCCCCACGCTTTCGTACCTGAGCGTCAGTCTTCGTCCAGGG1590.22618641174462273No Hit
CCTGTTCGCTACCCATGCTTTCGTGCCTCAGCGTCAGTTACAGACCAGAC1530.21765107545237283No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCTTGGCC1400.19915784681916468No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAA1370.19489017867303973No Hit
CCTGTTCGCTCCCCACGCTTTCGTTCCTCAGCGTCAGTAACGGCCCAGAG1310.1863548423807898No Hit
CCTGTTTGATCCCCACGCTTTCGCACCTCAGCGTCAGTTACAGACCAGAA1200.1707067258449983No Hit
CCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAG1190.16928416979628996No Hit
CCTGTTCGCTCCCCCAGCTTTCGTGCCTCAGCGTCGGTCTCGGCCCAGAG1170.16643905769887335No Hit
CCTGTTTGATCCCCACGCTTTCGCGCATCAGCGTCAGTAATGGCTTGGCA1070.15221349721179014No Hit
CCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1010.14367816091954022No Hit
CCTGTTCGCTCCCCCCGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG970.13798793672470694No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG970.13798793672470694No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGCGTCAGTATGATGCCAGGG950.13514282462729033No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAA810.11522703994537385No Hit
CCTGTTTGATCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG790.11238192784795721No Hit
CCTGTTTGCTACCCATGCTTTCGTTCCTCAGCGTCAGTAACTGCCCAGTA750.10669170365312393No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCCG152.003364E-5228.32445195
AAACCCG152.003364E-5228.32445195
CCAACCG100.001818732228.32445195
CAACCCG152.003364E-5228.32445195
AAGGGCG302.7284841E-11228.32445195
GCAACCG302.7284841E-11228.32445195
ATAACCA4400.0228.32445195
GAACCCG202.202105E-7228.32445195
AAGGCGG100.001818732228.32445195
AGACCCG100.001818732228.32445195
CGCACCG152.003364E-5228.32445195
AATGCCG152.003364E-5228.32445195
CGACCCG202.202105E-7228.32445195
ACAACCG400.0228.32445195
CAAACCA1300.0228.32443195
GAAACCG13300.0227.46608195
AAAACCG10750.0227.26247195
AAAGCCG10750.0225.13852195
CGAACCG3100.0224.64177195
CGGGCCG3450.0221.70633195
Produced by FastQC (version 0.11.5)