FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301799

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301799
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93581
Sequences flagged as poor quality0
Sequence length198-201
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA2249924.042273538431946No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGTA1202412.848762035028477No Hit
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGTA1149212.280270567743452No Hit
TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA76908.217480044026031No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA63426.777016702108334No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA58286.227759908528441No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA50665.413492055011167No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA47855.113217426614377No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA34413.677028456631154No Hit
TACGTAGGGTGCAAGCGTTGTCCGGAATTACTGGGCGTAAAGAGCTCGTA28993.097851059509943No Hit
TACGTAGGGGGCGAGCGTTATCCGGATTCATTGGGCGTAAAGCGCGCGTA20232.161763605860164No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA19042.034601040809566No Hit
TACGAAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA12141.297271882112822No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGTA1840.1966211089857984No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGAGCGCA1520.16242613351000737No Hit
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGCGCGTA1160.12395678609974246No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGCGTA1020.1089964843290839No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGG100.0022993335211.1743195
ACGGCGG152.7396585E-5211.1743195
ACGGCAG100.0022993335211.1743195
AAGGCAG34900.0209.96411195
AAGGCGG37200.0209.18744195
AAGGCGA11750.0207.57985195
GGTTGCA204.8586116E-7194.921457
TTGCAAG204.8586116E-7194.921459
ACGTCGG100.0029224516194.921452
AGGACCC100.0029224516194.921456
GGACCCG100.0029224516194.921457
GTCCCGA5950.0194.921458
AGGTCCC5950.0194.921456
GTAGGTC5950.0194.921454
TAGGGTG6100.0194.921455
TCCCGAG5950.0194.921459
GACCCGA100.0029224516194.921458
GTCGGTG100.0029224516194.921454
TAGGTCC5950.0194.921455
CGGAGGT308.185452E-11194.921433
Produced by FastQC (version 0.11.5)