FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301800

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301800
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93581
Sequences flagged as poor quality0
Sequence length197-201
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1978621.143180773875038No Hit
CCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAA1108111.841078851476261No Hit
CCTGTTTGCTCCCCATGCTTTCGTACCTCAGCGTCAGTATTAGGCCAGAT1097511.727807995212704No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGTGTCAGTATGATGCCAGGG73607.8648443594319355No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG58416.241651617315481No Hit
CCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAC49125.248928735533923No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG46885.009563907203385No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCTTGGCA43324.62914480503521No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG34793.717634990008656No Hit
CCTGTTTGCTACCCATGCTTTCGCTCCTCAGCGTCAGTAACTGCCCAGTA27822.972825680426582No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG20242.162832198843782No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA16061.7161603316912621No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT11231.2000299206035414No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGACCAGAG7780.8313653412551694No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCTTGGGA5850.6251268954168047No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG5230.5588741304324596No Hit
CCTGTTTGATCCCCACGCTTTCGTACATCAGCGTCAGTTACAGACCAGAA4590.4904841794808775No Hit
CCTGTTTGCTACCCACGCTTTCGTACCTCAGTGTCAGTATGATGCCAGGG2860.3056175933148823No Hit
CCTGTTTGCTCCCCATGCTTTCGCACCTCAGCGTCAGTATTAGGCCAGAT2730.29172588452784215No Hit
CCTGTTTGATCCCCACGCTTTCGCACCTCAGCGTCAGTTACAGACCAGAA2550.2724912108227097No Hit
CCTGTTCGCTACCCATGCTTTCGTGCCTCAGCGTCAGTTACAGACCAGAC2280.24363920026501104No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGACCAGAG2230.23829623534691874No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAA1850.19768970196941688No Hit
CCTGTTTGCTCCCCACGCTTTCGTACCTGAGCGTCAGTCTTCGTCCAGGG1610.17204347036257361No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG1490.15922035455915196No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCTTGGCC1460.15601457560829654No Hit
CCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAG1450.1549459826246781No Hit
CCTGTTCGCTCCCCACGCTTTCGTTCCTCAGCGTCAGTAACGGCCCAGAG1360.14532864577211185No Hit
CCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1310.13998568085401952No Hit
CCTGTTTGCTACCCATGCTTTCGTTCCTCAGCGTCAGTAACTGCCCAGTA1300.13891708787040105No Hit
CCTGTTCGCTCCCCCCGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG1240.13250552996869025No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGCGTCAGTATGATGCCAGGG1210.1292997510178348No Hit
CCTGTTTGATCCCCACGCTTTCGCGCATCAGCGTCAGTAATGGCTTGGCA1180.1260939720669794No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAA1080.1154080422307947No Hit
CCTGTTCGCTCCCCCAGCTTTCGTGCCTCAGCGTCGGTCTCGGCCCAGAG1030.11006507731270236No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACCG450.0230.72343195
AACGCCG100.0017637665230.72342195
AAACCCG302.5465852E-11230.72342195
CGGGGCG100.0017637665230.72342195
GCAACCG550.0230.72342195
CGGGCCG1600.0230.72342195
GAACCCG100.0017637665230.72342195
CGAACCG2850.0230.72342195
CGTACCG100.0017637665230.72342195
CGGCCCG151.9238509E-5230.72342195
TGAACCA850.0230.72342195
AATGCCG202.0944753E-7230.72342195
CAAACCA2300.0230.72342195
ATAACCA4550.0230.7234195
GAAACCG21300.0229.64021195
AAAACCG16700.0227.26947195
AAAGCCG13450.0226.43489195
AAGGCCG2550.0221.67545195
AAGGGCG550.0209.74857195
GACACCG450.0205.0875195
Produced by FastQC (version 0.11.5)