FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65609
Sequences flagged as poor quality0
Sequence length198-201
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA1333120.318858693167098No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGTA857313.066804859089453No Hit
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGTA803412.245271228032738No Hit
TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA55998.533890167507506No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA48577.402947766312548No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA43646.651526467405386No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA40546.179030315962749No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA29604.511576155710344No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA26304.008596381593989No Hit
TACGTAGGGTGCAAGCGTTGTCCGGAATTACTGGGCGTAAAGAGCTCGTA21493.2754652562910573No Hit
TACGTAGGGGGCGAGCGTTATCCGGATTCATTGGGCGTAAAGCGCGCGTA18772.860887987928485No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA14342.1856757457056197No Hit
TACGAAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA11131.6964136017924372No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGTA1450.22100626438445944No Hit
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGCGCGTA1020.15546647563596458No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGAGCGCA860.13107957749698976No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA840.12803121522961788No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGCGTA690.10516849822432897No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGCAG25950.0211.81766195
AAGGCGG24900.0209.66962195
AAGGCGA7800.0206.78485195
CGGAGGT100.0029206031194.918463
CGGAGGA6400.0194.918463
GGGCAAG100.0029206031194.918469
GGCGCGA100.0029206031194.918468
AGGATCC6400.0194.918466
GGTCCCG4800.0194.918467
ACGTATG153.7667283E-5194.918462
GCGCGAG100.0029206031194.918469
GTCCCGA4800.0194.918468
TACGTAT153.7667283E-5194.918461
CGTATGT100.0029206031194.918463
TACGAAG950.0194.918461
AGGTCCC4850.0194.918466
GTAGGGC100.0029206031194.918464
TCCGTAG100.0029206031194.918461
GGGGCAA100.0029206031194.918468
TCCCGAG4800.0194.918469
Produced by FastQC (version 0.11.5)