FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301802

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301802
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65609
Sequences flagged as poor quality0
Sequence length198-201
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1177217.942660305750735No Hit
CCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAA827112.606502156716303No Hit
CCTGTTTGCTCCCCATGCTTTCGTACCTCAGCGTCAGTATTAGGCCAGAT773811.794113612461706No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGTGTCAGTATGATGCCAGGG54948.373851148470484No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG43576.640857199469585No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG39516.022039659193099No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCTTGGCA32444.9444435976771475No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG29044.426222012223933No Hit
CCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAC25563.89580697770123No Hit
CCTGTTTGCTACCCATGCTTTCGCTCCTCAGCGTCAGTAACTGCCCAGTA20973.1962078373393896No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG19402.956911399350699No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA12201.859500983096831No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT10621.618680363974455No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCTTGGGA4670.7117925894313281No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGACCAGAG4450.6782606044902376No Hit
CCTGTTTGATCCCCACGCTTTCGTACATCAGCGTCAGTTACAGACCAGAA3350.5106006797847856No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG2610.397811275892027No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGACCAGAG1900.28959441540032616No Hit
CCTGTTTGCTCCCCATGCTTTCGCACCTCAGCGTCAGTATTAGGCCAGAT1770.2697800606624091No Hit
CCTGTTTGATCCCCACGCTTTCGCACCTCAGCGTCAGTTACAGACCAGAA1680.25606243045923577No Hit
CCTGTTTGCTACCCACGCTTTCGTACCTCAGTGTCAGTATGATGCCAGGG1590.24234480025606245No Hit
CCTGTTTGCTCCCCACGCTTTCGTACCTGAGCGTCAGTCTTCGTCCAGGG1500.22862717005288907No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCTTGGCC1280.19509518511179869No Hit
CCTGTTCGCTCCCCACGCTTTCGTTCCTCAGCGTCAGTAACGGCCCAGAG1210.18442591717599718No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG1170.1783291926412535No Hit
CCTGTTCGCTACCCATGCTTTCGTGCCTCAGCGTCAGTTACAGACCAGAC1080.16461156243808014No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAA1050.16003901903702236No Hit
CCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAG950.14479720770016308No Hit
CCTGTTTGCTACCCATGCTTTCGTTCCTCAGCGTCAGTAACTGCCCAGTA890.1356521208980475No Hit
CCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG890.1356521208980475No Hit
CCTGTTCGCTCCCCCCGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG860.13107957749698976No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGCGTCAGTATGATGCCAGGG810.12345867182856012No Hit
CCTGTTCGCTCCCCCAGCTTTCGTGCCTCAGCGTCGGTCTCGGCCCAGAG780.11888612842750233No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAGTAATGGCTTGGCA750.11431358502644454No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAA730.11126522275907269No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGCG450.0231.96645195
GCAACCG302.3646862E-11231.96643195
CGGGCCG2350.0231.96643195
AAACCCG202.0335574E-7231.96642195
GAACCCG252.2100721E-9231.96642195
CGAACCG3550.0231.96642195
AAAGGCG202.0335574E-7231.96642195
CAAACCA950.0231.9664195
AAAACCG10450.0229.74666195
GAAACCG13600.0229.40797195
AAAGCCG9050.0229.40327195
ATAACCA3700.0228.83176195
AAGGCCG2350.0227.03098195
TGAACCA600.0212.6359195
ATCCCCA14000.0194.837669
GTTTGCT35250.0194.837664
GTTTGAT15450.0194.837664
GATACCC1450.0194.837668
TGATCCC14050.0194.837667
TGTTTGC35200.0194.837663
Produced by FastQC (version 0.11.5)